Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR.

Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR. Research Abstract Details 

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Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR. Abstract Text:

Christopher W M Kay,Celine ,Robert Bittl,Scott R Farrell,Colin Thorpe,

Pulsed electron-electron double resonance (ELDOR) has been used to obtain structural information from a FAD-dependent sulfhydryl oxidase, Augmenter of Liver Regeneration (ALR). ALR is a homodimer with each subunit containing a noncovalently bound FAD cofactor. Both FADs may be converted into the blue neutral radical form by aerobic treatment with DTT. From three-pulse and four-pulse ELDOR experiments, a distance of 26.1 +/- 0.8 A could be determined between the FAD cofactors in human ALR. Taking into account the electron spin density distribution in a neutral flavin radical obtained from density functional theory calculations, a distance of 26.9 A could be estimated for the separation of the spin centers in the X-ray structure of rat ALR. The good agreement confirms that rat ALR may be used as a model for mechanistic discussions of human ALR. The experiments also demonstrate that neutral flavin radicals have the appropriate properties to be used as intrinsic spin labels for distance determinations in proteins.

Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR. Publishing Authors By Initials

CW Kay,C ,R Bittl,SR Farrell,C Thorpe,

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Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 128

Page Numbers: 76-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 11

MONTH: Jan

YEAR: 2006

Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR. Information

Substance Name: sulfhydryl oxidase

Registry Number: EC 1.8.3.-

Grant and Affiliation Information for Determination of the distance between the two neutral flavin radicals in augmenter of liver regeneration by pulsed ELDOR.

AFFILIATION: Institut für Experimentalphysik, Fachbereich Physik, Freie Universität Berlin, 14195 Berlin, Germany. chris.kay@physik.fu-berlin.de

Country: United States

AGENCY: United States NIGMS

GRANT: GM 26643

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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