Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study.

Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study. Research Abstract Details 

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Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study. Abstract Text:

An interlaboratory validation study funded by the European Commission, Directorate General for Health and Consumer Protection (DG SANCO), was conducted to evaluate the effectiveness of a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for the determination of semicarbazide (SEM) in different types of baby food at a possible future European regulatory limit (10 ng/g). The test portion of the sample was extracted with hydrochloric acid, and the analyte was derivatized with 2-nitrobenzaldehyde, with 1,2-[15N2, 13C] SEM as an internal standard. The extract was neutralized and then purified on a solid-phase extraction cartridge. The SEM was determined by reversed-phase LC with detection by MS/MS. Apple puree, rice pudding, and meat/vegetable meal baby food materials, spiked with SEM at levels of about 3, 10, and 30 ng/g, respectively, were sent to 20 laboratories in 12 different European countries, which submitted results from 17 participants. Recoveries ranged from 88.8 to 106.1%. Based on results for spiked samples (blind pairs at 3 levels), the relative standard deviations for repeatability (RSDr) ranged from 4.2 to 6.9% and the relative standard deviations for reproducibility (RSDR) ranged from 16.6 to 24.3%. The method showed acceptable within- and between-laboratory precision for all 3 matrixes, as evidenced by HorRat values, at the target levels for the determination of SEM.

Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study. Publishing Authors By Initials

For similar organic chemicals: semicarbazides research abstracts see: organic chemicals: semicarbazides research

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Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of AOAC International

VOLUME: 89

Page Numbers: 728-34

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study. Information

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LANGUAGE: eng

NlmUniqueID: 9215446

Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study. Keywords Mesh Terms:

KEYWORDS: Semicarbazides

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study. Information

Substance Name: Hydrochloric Acid

Registry Number: 7647-01-0

Grant and Affiliation Information for Determination of semicarbazide in baby food by liquid chromatography/tandem mass spectrometry: interlaboratory validation study.

AFFILIATION: Central Science Laboratory (CSL), Sand Hutton, York, YO41 lLZ, United Kingdom.

Country: United States

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MEDLINETA: J AOAC Int

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