Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1.

Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1. Research Abstract Details 

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Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1. Abstract Text:

YanMing Zou,HongJun Wang,Jason L Shapiro,Curtis T Okamoto,Steven J Brookes,S Petter Lyngstadaas,Malcolm L Snead,Michael L Paine,YanMing Zou,HongJun Wang,Jason L Shapiro,Curtis T Okamoto,Steven J Brookes,S Petter Lyngstadaas,Malcolm L Snead,Michael L Paine,

The enamel matrix protein amelogenin is secreted by ameloblasts into the extracellular space to guide the formation of highly ordered hydroxyapatite mineral crystallites, and, subsequently, is almost completely removed during mineral maturation. Amelogenin interacts with the transmembrane proteins CD63 and LAMP (lysosome-associated membrane protein) 1, which are involved in endocytosis. Exogenously added amelogenin has been observed to move rapidly into CD63/LAMP1-positive vesicles in cultured cells. In the present study, we demonstrate the protein region defined by amino acid residues 103-205 for CD63 interacts not only with amelogenin, but also with other enamel matrix proteins (ameloblastin and enamelin). A detailed characterization of binding regions in amelogenin, CD63 and LAMP1 reveals that the amelogenin region defined by residues PLSPILPELPLEAW is responsible for the interaction with CD63 through residues 165-205, with LAMP1 through residues 226-251, and with the related LAMP2 protein through residues 227-259. We predict that the amelogenin binding region is: (i) hydrophobic; (ii) largely disordered; and (iii) accessible to the external environment. In contrast, the binding region of CD63 is likely to be organized in a '7' shape within the mushroom-like structure of CD63 EC2 (extracellular domain 2). In vivo, the protein interactions between the secreted enamel matrix proteins with the membrane-bound proteins are likely to occur at the specialized secretory surfaces of ameloblast cells called Tomes' processes. Such protein-protein interactions may be required to establish short-term order of the forming matrix and/or to mediate feedback signals to the transcriptional machinery of ameloblasts and/or to remove matrix protein debris during enamel biomineralization.

Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1. Publishing Authors By Initials

Y Zou,H Wang,JL Shapiro,CT Okamoto,SJ Brookes,SP Lyngstadaas,ML Snead,ML Paine,Y Zou,H Wang,JL Shapiro,CT Okamoto,SJ Brookes,SP Lyngstadaas,ML Snead,ML Paine,

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Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Biochemical journal

VOLUME: 408

Page Numbers: 347-54

Journal Abbreviation: Biochem. J.

ISSN: 1470-8728

DAY: 15

MONTH: Dec

YEAR: 2007

Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1. Information

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LANGUAGE: eng

NlmUniqueID: 2984726

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Grant and Affiliation Information for Determination of protein regions responsible for interactions of amelogenin with CD63 and LAMP1.

AFFILIATION: University of Southern California School of Dentistry, Center for Craniofacial Molecular Biology, 2250 Alcazar Street, CSA Room 103, Los Angeles, CA 90033-1004, USA.

Country: England

AGENCY: United States NIDCR

GRANT: DE014867

ACRONYM: DE

MEDLINETA: Biochem J

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