Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer.

Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer. Research Abstract Details 

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Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer. Abstract Text:

Hiroyuki Yamada,Shinjiro Matsuda,Yoshihiro Ushio,Kenji Nakamura,Shinzo Kobatake,Shinji Satomura,Shuji Matsuura,Hiroyuki Yamada,Shinjiro Matsuda,Yoshihiro Ushio,Kenji Nakamura,Shinzo Kobatake,Shinji Satomura,Shuji Matsuura,

BACKGROUND: Procalcitonin (PCT) is a biomarker for the diagnosis of sepsis and bacterial infection diseases. METHODS: A new fully automated SphereLight PCT (SL-PCT) assay system for PCT concentration in human serum or plasma by using SphereLight 180 (SL180, Olympus Corp.) analyzer was developed. The SL-PCT assay is based on chemiluminescent enzyme immunoassay. RESULTS: A linear dose response relationship was observed up to 200 ng/ml PCT concentration. The detection limit of PCT concentration was 0.06 ng/ml. Endogenous substances, anticoagulants, sodium fluoride and drugs did not interfere with assay results. There was a good correlation between the present method and the manual method in serum and plasma samples. CONCLUSIONS: These results indicate that the SL-PCT assay showed good performance in terms of the linearity, detection limit and precision. Use of this PCT measurement may improve the detection of sepsis and infectious disease.

Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer. Publishing Authors By Initials

H Yamada,S Matsuda,Y Ushio,K Nakamura,S Kobatake,S Satomura,S Matsuura,H Yamada,S Matsuda,Y Ushio,K Nakamura,S Kobatake,S Satomura,S Matsuura,

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Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinica chimica acta; international journal of cli

VOLUME: 388

Page Numbers: 38-40

Journal Abbreviation: Clin. Chim. Acta

ISSN: 0009-8981

DAY: 6

MONTH: 10

YEAR: 2007

Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer. Information

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LANGUAGE: eng

NlmUniqueID: 1302422

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Grant and Affiliation Information for Determination of procalcitonin concentration using the SphereLight 180 clinical auto-analyzer.

AFFILIATION: Osaka Research Laboratories, Wako Pure Chemical Industries, Ltd 6-1 Takada-cho, Amagasaki, Hyogo 66-0963, Japan.

Country: Netherlands

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MEDLINETA: Clin Chim Acta

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