[Determination of plasma homocysteine in normal adults by high performance liquid chromatography with fluorescence detection]

[Determination of plasma homocysteine in normal adults by high performance liquid chromatography with fluorescence detection] Research Abstract Details 

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[Determination of plasma homocysteine in normal adults by high performance liquid chromatography with fluorescence detection] Abstract Text:

M Li,Y Wang,X Zhu,J Zheng,

A rapid, isocratic high performance liquid chromatographic(HPLC) method with fluorescence detection was used for the determination of total homocysteine (Hcy) levels in human plasma. Prior to reversed-phase HPLC analysis, the plasma thiols were derivatized with ABDF(7-fluoroben30-2-oxa-1,3-diazole-4-sulfonamide), a thiol specific fluorogenic probe. A mobile phase consisted of 0.1 mol/L potassium dihydrogen phosphate (pH 2.1, containing 10% of acetonitrile) ensured base line separation of plasma Hcy. The mean recovery of this method was 97.75%. The intraday and interday coefficient of variations were 4.49% and 9.79%, respectively. The method is simple, sensitive, reproducible and very suitable for routine determination of plasma Hcy in human.

[Determination of plasma homocysteine in normal adults by high performance liquid chromatography with fluorescence detection] Publishing Authors By Initials

M Li,Y Wang,X Zhu,J Zheng,

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[Determination of plasma homocysteine in normal adults by high performance liquid chromatography with fluorescence detection] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Se pu = Chinese journal of chromatography / Zhongg

VOLUME: 17

Page Numbers: 310-2

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ISSN: 1000-8713

DAY: 28

MONTH: May

YEAR: 1999

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LANGUAGE: chi

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AFFILIATION: Third Hospital of Suzhou, Research Center for Digestive Disease and Nutrition of Suzhou, Suzhou, 215008.

Country: China

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MEDLINETA: Se Pu

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