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Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography.

Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography. Research Abstract Details 

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  • Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography. Abstract Text:

    A simultaneous determination of orotic acid, uric acid, and creatinine in milk is described. Following deproteinization, the sample was analyzed by reversed-phase liquid chromatography, using a highly aqueous cationic ion-pair eluent and photodiode array UV detection. In view of their potential dietary significance, the validated method was applied to survey the influence of species, season, and lactation on their contribution to the nonprotein nitrogen pool in milk. Mature bovine milk contained orotic acid, uric acid, and creatinine in the range of 30-70, 9-24, and 6-12 microg/mL, respectively. Although uric acid and creatinine were present in all milks, orotic acid was essentially absent in nonruminant milks. In contrast to urate and creatinine, expression of orotic acid in bovine milk was strongly dependent on stage of early lactation. The co-existence in mammalian milks of related nucleoside and nucleotide components was also determined.

    Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography. Publishing Authors By Initials

    For similar heterocyclic compounds: alkaloids: xanthines: uric acid research abstracts see: heterocyclic compounds: alkaloids: xanthines: uric acid research

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    Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Journal of AOAC International

    VOLUME: 87

    Page Numbers: 116-22

    Journal Abbreviation: J AOAC Int

    ISSN: 1060-3271

    DAY: 17

    MONTH: 03

    YEAR: 2008

    Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 9215446

    Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography. Keywords Mesh Terms:

    KEYWORDS: Uric Acid

    MESH TERMS: analysis

    Chemical & Substance for Abstract: Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography. Information

    Substance Name: Uric Acid

    Registry Number: 69-93-2

    Grant and Affiliation Information for Determination of orotic acid, uric acid, and creatinine in milk by liquid chromatography.

    AFFILIATION: Fonterra, No. 1 Rd, PO Box 7, Waitoa, New Zealand. harvey.indyk@fonterra.com

    Country: United States

    United States Research PublicationUnited States Research Publication

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    MEDLINETA: J AOAC Int

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