Determination of nimodipine in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and pharmacokinetic application.

Determination of nimodipine in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and pharmacokinetic application. Research Abstract Details 

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Determination of nimodipine in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and pharmacokinetic application. Abstract Text:

Feng Qin,Yuanyuan Ma,Yiqi Wang,Lingyun Chen,Dong Wang,Famei Li,

A fast and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination and pharmacokinetic study of nimodipine in human plasma. With nitrendipine as the internal standard, sample pretreatment involved one-step extraction with diethyl ether of 0.5ml plasma. The separation was carried out on an ACQUITY UPLCtrade mark BEH C(18) column (50mmx2.1mm, i.d., 1.7mum) with water and acetonitrile (both containing 0.1% formic acid) as the mobile phase under gradient conditions at a flow rate of 0.35ml/min. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode via electrospray ionization (ESI) source. The standard curves were linear (r(2)>/=0.99) over the concentration range of 0.20-100ng/ml with a limit of quantification (LLOQ) of 0.20ng/ml. The intra- and inter-day precision (R.S.D.) values were below 14% and the accuracy (relative error R.E.) was ranged from -2.2% to 7.7% at all three quality control (QC) levels. The method herein described was superior to previous methods and successfully applied to the pharmacokinetic study of nimodipine tablets in healthy male volunteers after oral administration.

Determination of nimodipine in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and pharmacokinetic application. Publishing Authors By Initials

F Qin,Y Ma,Y Wang,L Chen,D Wang,F Li,

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Determination of nimodipine in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and pharmacokinetic application. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of pharmaceutical and biomedical analysis

VOLUME: 46

Page Numbers: 557-62

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ISSN: 0731-7085

DAY: 9

MONTH: 11

YEAR: 2007

Determination of nimodipine in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and pharmacokinetic application. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Determination of nimodipine in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and pharmacokinetic application.

AFFILIATION: Department of Analytical Chemistry, Shenyang Pharmaceutical University, Wenhua Road 103#, Shenyang 110016, PR China.

Country: England

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MEDLINETA: J Pharm Biomed Anal

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