Determination of neomycin in animal tissues by liquid chromatography.

Determination of neomycin in animal tissues by liquid chromatography. Research Abstract Details 

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Determination of neomycin in animal tissues by liquid chromatography. Abstract Text:

Tissue samples are digested under hot alkaline conditions after initial conditioning at room temperature with phosphate-buffered saline. The cooled digest is deproteinated with concentrated perchloric acid. After centrifugation and pH adjustment, the clear supernatant is applied to an ion-exchange cartridge, and after the cartridge is washed, the neomycin is eluted with dilute perchloric acid. This eluate is derivatized with 9-fluorenylmethyl chloroformate prior to liquid chromatography using a wide-pore spherical silica C4 column and fluorescence detection. Recovery and repeatability are calculated from tissue extract standard calibration curves produced from the same assay. Recoveries ranged from 80 to 120% for fortifications of 0.25-1.00 mg/kg for muscle tissue and from 80 to 100% for fortifications of 0.50-10.0 mg/kg for kidney tissue. Limits of quantitation were 0.25 and 0.50 mg/kg, respectively, for muscle and kidney tissues. Limits of detection were 0.125 and 0.20 mg/kg, respectively, for muscle and kidney tissues.

Determination of neomycin in animal tissues by liquid chromatography. Publishing Authors By Initials

For similar complex mixtures: tissue extracts research abstracts see: complex mixtures: tissue extracts research

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Determination of neomycin in animal tissues by liquid chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 82

Page Numbers: 61-7

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Determination of neomycin in animal tissues by liquid chromatography. Information

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LANGUAGE: eng

NlmUniqueID: 9215446

Determination of neomycin in animal tissues by liquid chromatography. Keywords Mesh Terms:

KEYWORDS: Tissue Extracts

MESH TERMS: chemistry

Chemical & Substance for Abstract: Determination of neomycin in animal tissues by liquid chromatography. Information

Substance Name: Neomycin

Registry Number: 1404-04-2

Grant and Affiliation Information for Determination of neomycin in animal tissues by liquid chromatography.

AFFILIATION: Canadian Food Inspection Agency, Centre for Veterinary Drug Residues, Saskatoon, Saskatchewan, Canada.

Country: UNITED STATES

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MEDLINETA: J AOAC Int

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