Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant.

Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant. Abstract Text:

S Maezawa,Y Hayashi,T Nakae,J Ishii,K Kameyama,T Takagi,

An assessment study was carried out to evaluate the performance of the low-angle laser light scattering technique combined with high-performance gel chromatography in the presence of a nonionic surfactant, octaethyleneglycol n-dodecyl ether, precision differential refractometry and ultraviolet photometry. It was found that the combined technique is highly promising as a method for the determination of the molecular weight of a membrane protein solubilized by the surfactant. For trial, molecular weights of the following membrane proteins of Escherichia coli, both solubilized in oligomeric forms, were measured; porin that forms the transmembrane diffusion pore in the outer membrane, and lambda-receptor protein that facilitates the diffusion of maltose-maltodextrins across the outer membrane. The result obtained indicates that both porin and lambda-receptor protein exist as trimers in the surfactant solution.

Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant. Publishing Authors By Initials

S Maezawa,Y Hayashi,T Nakae,J Ishii,K Kameyama,T Takagi,

For similar chemical actions and uses: specialty uses of chemicals: surface-active agents research abstracts see: chemical actions and uses: specialty uses of chemicals: surface-active agents research

PUBMED ID PMID:

MEDLINE DATE:

Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimica et biophysica acta

VOLUME: 747

Page Numbers: 291-7

Journal Abbreviation: Biochim. Biophys. Acta

ISSN: 0006-3002

DAY: 28

MONTH: Sep

YEAR: 1983

Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 217513

Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant. Keywords Mesh Terms:

KEYWORDS: Surface-Active Agents

MESH TERMS: analysis

Chemical & Substance for Abstract: Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant. Information

Substance Name: maltoporins

Registry Number: 0

Grant and Affiliation Information for Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant.

AFFILIATION:

Country: NETHERLANDS

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biochim Biophys Acta

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant Related Publications

• Administration of hematopoietic cytokines increases the expression of anti-inflammatory cytokine (IL-10) mRNA in the subacute phase after stroke.
• An extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4, isolated from crude oil.
• Antimicrobial Principles of Xian he cao (Agrimonia pilosa).
• Blue light-dependent nuclear positioning in Arabidopsis thaliana leaf cells.
• Comparative laboratory study on the reaction of Aedes aegypti and Aedes albopictus to different attractive cues in a mosquito trap.
• Comparison of 1-day, 2-day, and 3-day administration of antimicrobial prophylaxis in radical prostatectomy.
• Desensitization of feedback inhibition of the Saccharomyces cerevisiae gamma-glutamyl kinase enhances proline accumulation and freezing tolerance.
• Determination of molecular weight of membrane proteins by the use of low-angle laser light scattering combined with high-performance gel chromatography in the presence of a non-ionic surfactant.
• Determination of phosphorylated amino acid residues of Rab8 from Bombyx mori.
• Dynamic processes in endocytic transformation of a raft-exhibiting giant liposome.
• Efficient production of 2-deoxy-scyllo-inosose from d-glucose by metabolically engineered recombinant Escherichia coli.
• Gene expression of bone matrix proteins in a calcified tissue appeared in subcutaneously transplanted rat dental pulp.
• Glucocorticoids induce the differentiation of a mesenchymal progenitor cell line, ROB-C26 into adipocytes and osteoblasts, but fail to induce terminal osteoblast differentiation.
• Immunohistochemical analysis of centromere protein F expression in buccal and gingival squamous cell carcinoma.
• Inductive effects of dexamethasone on the mineralization and the osteoblastic gene expressions in mature osteoblast-like ROS17/2.8 cells.
• Interaction between Helicobacter pylori and immune response to CagA: CagA antibody may down-regulate bacterial colonization and tyrosine phosphorylation.
• Interspecific variation in diving activity among Anopheles gambiae Giles, An. arabiensis Patton, and An. funestus Giles (Diptera: Culicidae) larvae.
• Isoflavones of Belamcanda chinensis.
• Local tunneling spectroscopy across a metamagnetic critical point in the bilayer ruthenate Sr3Ru2O7.
• Modelling HA protein-mediated interaction between an influenza virus and a healthy cell: pre-fusion membrane deformation.
• Overexpression of two transcriptional factors, Kin28 and Pog1, suppresses the stress sensitivity caused by the rsp5 mutation in Saccharomyces cerevisiae.
• Reversible redox processes of poly(anilines) in layered semiconductor niobate films under alternate UV-vis light illumination.
• Sarothralen A and B, New Antibiotic Compounds from Hypericum japonicum.
• Spin-liquid state in the S=1/2 hyperkagome antiferromagnet Na4Ir3O8.
• Structural basis for ligand recognition by integrins.
• Studies on Iridoid-related Compounds III: Gentiopicral, the Aglucone of Gentiopicroside.
• Sudden death due to undiagnosed acute promyelocytic leukemia: a case report.
• The PLEXIN PLX-2 and the ephrin EFN-4 have distinct roles in MAB-20/Semaphorin 2A signaling in Caenorhabditis elegans morphogenesis.
• Zirconocene-mediated highly regio- and stereoselective synthesis of multisubstituted olefins starting from 1-alkynylboronates.
• [Ischemic brain injury and hepatocyte growth factor]