[Determination of metronidazole in gastric juice by high performance liquid chromatography]

[Determination of metronidazole in gastric juice by high performance liquid chromatography] Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

[Determination of metronidazole in gastric juice by high performance liquid chromatography] Abstract Text:

G He,D Tan,C Zhong,

Metronidazole (MTZ) is the first chosen drug of anti-anaerobe which was recommended by World Health Organization. In this paper a high performance liquid chromatographic (HPLC) method has been developed to determine the concertration of MTZ in human gastric juice. Gastric juice was obtained by intubation after taking drug, then the sample was precipitated with ethanol to remove proteins, centrifuged, filtered and finally assayed by HPLC. The operating conditions were reversed phase C18 column (4mm x 150mm), CH3OH:KH2PO4 (5mmol/L, pH 4.0) = 10:90 mobile phase with flow rate of 1.5mL/min and detection wavelength at 320nm. The standard working curve was Y = -1.63 + 1.15X, with r = 0.9999, n = 7. The linear range was 2.5-200mg/L. The detection limit was 0.4mg/L. The average recovery was 96.0% and the coefficient of variation was 3.07%. This method can be applied to guide clinical therapy and pharmaceutical research.

[Determination of metronidazole in gastric juice by high performance liquid chromatography] Publishing Authors By Initials

G He,D Tan,C Zhong,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

[Determination of metronidazole in gastric juice by high performance liquid chromatography] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Se pu = Chinese journal of chromatography / Zhongg

VOLUME: 15

Page Numbers: 228-30

Journal Abbreviation:

ISSN: 1000-8713

DAY: 2

MONTH: May

YEAR: 1997

[Determination of metronidazole in gastric juice by high performance liquid chromatography] Information

Number of References:

LANGUAGE: chi

NlmUniqueID: 9424804

[Determination of metronidazole in gastric juice by high performance liquid chromatography] Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: [Determination of metronidazole in gastric juice by high performance liquid chromatography] Information

Substance Name:

Registry Number:

Grant and Affiliation Information for [Determination of metronidazole in gastric juice by high performance liquid chromatography]

AFFILIATION: The Laboratory of Molecular Medicine, Sun Yat-sen University of Medical Sciences, Guangzhou, 510089.

Country: China

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Se Pu

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Determination of metronidazole in gastric juice by high performance liquid chromatography Related Publications

• Expression and purification of recombinant bone morphogenetic protein-2 in E.coli.
• [A clinical analysis of 30 cases of sympathetic ophthalmia]
• [An epidemiologic study of cough in young college students in Guangzhou]
• [CD4+CD25+ T lymphocytes in peripheral blood from patients with asthma]
• [Clinical observation on liang's anti-rheumatism and rheumatoid granule in rheumatoid arthritis in the active stage]
• [Deep fungal infections: new challenges and prospective]
• [Detection and related analysis to chromosome 22q11 microdeletion in patients with congenital heart diseases]
• [Determination of atomization efficiency in graphite furnace with V-boat]
• [Determination of metronidazole in gastric juice by high performance liquid chromatography]
• [Disparity of asthma prevalence in Chinese schoolchildren is due to differences in lifestyle factors]
• [Etiology and a diagnostic protocol for patients with chronic cough]
• [Expiratory flow limitation detected by negative expiratory pressure in patients with chronic obstructive pulmonary disease]
• [Expressions of L-type calcium channel and potassium channel Kv4.3 in rapid paced primary cultured atrial myocytes]
• [Finite element analysis of lumbar facet joint contact model]
• [Fluorescence spectroscopic analysis of huminite of the peat and soft brown coal]
• [Influence of extraction on fluorescence of the subrenite in the peat]
• [Inhibitory effect of grape procyanidin on the cell apoptosis induced by radiation]
• [Investigation of proximal femoral marrow with magnetic resonance imaging in recovered patients with severe acute respiratory syndrome]
• [Observation of the effect of hyperoxic sodium chloride solution on hypoxic injury]
• [Progress in the studies on hydrogel burn dressings]
• [Protective effect of specific antibody in serum of convalescent patient with SARS]
• [Research on quantified diagnosis and combining diseases with syndrome of blood stasis]
• [Studies on pathogenesis of primary biliary cirrhosis]
• [Subcutaneous implantation of tissue-engineered heart valve leaflets in nude mice]
• [The advance of research for biocompatibility of medical polyurethanes]
• [The epidemiology and management of influenza]
• [The protective effect of Rhadiola Astragalus Codonopsis Compound on myocardium in early stage of severe burn at plateau in rats]
• [The relationship between mouth pressure or tracheal pressure and esophagus pressure and diaphragmatic pressure]
• [Time trends in the prevalence of asthma and allergic diseases over 7 years among adolescents in Guangzhou city]
• [Uprighting of mesially or horizontally impacted mandibular molars]