Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay.

Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay. Abstract Text:

A benomyl polyclonal enzyme immunoassay (EIA) commercial kit was used to quantitate methyl 2-benzimidazolecarbamate (MBC), a degradation product of benomyl in wine. Total analysis time, including sample preparation, was 30 min. As many as 8 samples can be analyzed simultaneously with a limit of quantitation of 5 ppb. The assay logarithmic response was linear from 0.4 to 26 ppb MBC. Intra-assay percent coefficients of variation (%CVs) ranged from 2.4 to 13 for standards and from 7.4 to 21 for actual wine samples. Interassay %CVs varied from 2.6 to 15 for the standards and from 6.9 to 23 for the samples. Average recovery from samples spiked at 10-10,000 ppb was 93% for evaporated red and white wines. MBC was determined in 134 different wines by immunoassay and liquid chromatography (LC). Of these samples, 98 were positive for MBC by both methods with a correlation coefficient (r) of 0.986. The other 36 samples had MBC levels that either were not detectable by either procedure or were below the 10 ppb limit of quantitation for LC. Concentrations of MBC in wine ranged from 5 to 1329 ppb, with the majority ranging from 10 to 300 ppb. Also, a mini-study was conducted using the plate EIA format.

Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay. Publishing Authors By Initials

For similar food and beverages: beverages: alcoholic beverages: wine research abstracts see: food and beverages: beverages: alcoholic beverages: wine research

PUBMED ID PMID:

MEDLINE DATE:

Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of AOAC International

VOLUME: 76

Page Numbers: 851-6

Journal Abbreviation: J AOAC Int

ISSN: 1060-3271

DAY: 17

MONTH: 03

YEAR: 2008

Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9215446

Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay. Keywords Mesh Terms:

KEYWORDS: Wine

MESH TERMS: analysis

Chemical & Substance for Abstract: Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay. Information

Substance Name: mecarzole

Registry Number: 10605-21-7

Grant and Affiliation Information for Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay.

AFFILIATION: University of Maine, Department of Food Science, Orono 04469-5736.

Country: UNITED STATES

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J AOAC Int

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay Related Publications

• Constitution of ferrimycin A1.
• Determination of methyl 2-benzimidazolecarbamate in wine by competitive inhibition enzyme immunoassay.
• Evacuation of a 14-vertebral-level cervico-thoracic epidural abscess and review of surgical options for extensive spinal epidural abscesses.
• Fear, an important etiological factor in obstetric problems.
• Laser-induced damage and ion emission of GaAs at 1.06 mu.
• Percutaneous management of bile duct injury after laparoscopic cholecystectomy.
• Rheumatoid arthritis.
• Spontaneous hepatic hemorrhage in older women.
• [Basilar impression of cranium and pituitary function.]
• [Endocrine therapy of the metastasizing carcinoma of the breast.]
• [Experience in rearing colostrum-deprived piglets]
• [Inhibition of the secretion of the adrenal cortex induced by 9 alpha-fluorohydrocortisone.]
• [Melancholia from the medical viewpoint]
• [Not Available.]
• [Not Available.]
• [The therapy of encephalomalacia with streptokinase-streptodornase.]