Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection.

Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection. Research Abstract Details 

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Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection. Abstract Text:

Martin Novotny,Veronika ,Erica A Wehrwein,David L Kreulen,Greg M Swain,

Capillary electrophoresis with end-column amperometric detection (CE-EC) was used to determine the regional distribution of norepinephrine (NE) in the hearts of sympathetically innervated (control) and chemically sympathectomized rats. Key features of the method are (i) the sample preparation and clean-up step that involved the application of off-line solid phase extraction (SPE) with a 95% NE recovery and (ii) the use of a diamond microelectrode for detection. NE was quantified in the left and right ventricle, the ventricular septum, and the left and right atrium. The NE concentration in the atria was three to five times higher than in the ventricles and ventricular septum of control rats. Basal NE levels in the left and right ventricle and the ventricular septum were reduced to below the detection limit (0.034 microg/g tissue) in tissues treated with the neurotoxin, 6-hydroxydopamine (6-OHDA), while only a moderate reduction was observed in the left and right atrium. Importantly, the diamond microelectrode provided low and stable background current and low peak-to-peak noise

Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection. Publishing Authors By Initials

M Novotny,V ,EA Wehrwein,DL Kreulen,GM Swain,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research

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Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of neuroscience methods

VOLUME: 163

Page Numbers: 52-9

Journal Abbreviation:

ISSN: 0165-0270

DAY: 16

MONTH: 02

YEAR: 2007

Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection. Information

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LANGUAGE: eng

NlmUniqueID: 7905558

Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection. Keywords Mesh Terms:

KEYWORDS: Rats, Sprague-Dawley

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection. Information

Substance Name: Norepinephrine

Registry Number: 51-41-2

Grant and Affiliation Information for Determination of endogenous norepinephrine levels in different chambers of the rat heart by capillary electrophoresis coupled with amperometric detection.

AFFILIATION: Department of Chemistry and the Neuroscience Program, Michigan State University, 320 Chemistry Building, East Lansing, MI 48824-1322, USA.

Country: Netherlands

AGENCY: United States NHLBI

GRANT: HL70687

ACRONYM: HL

MEDLINETA: J Neurosci Methods

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