The application of reversed-phase gradient high-performance liquid chromatography with UV detection to the determination of cortisol and cortisone in 24-h urine samples is described. The method employs Sep-pak C18 cartridges for the part-purification and concentration of the corticosteroids, with sample enrichment at the head of an HPLC pre-column and separation using water/acetonitrile gradient. The internal standard is 6alpha-methylprednisolone. Measurement of both cortisone and cortisol provides further information on adrenocortical function. 24-hour excretion rate data from normal subjects are reported.
Determination of cortisol and cortisone in urine using high-performance liquid chromatography with UV detection. Publishing Authors By Initials
Determination of cortisol and cortisone in urine using high-performance liquid chromatography with UV detection. Journal Published:
PUBLICATION TYPE: Journal Article
Journal: Journal of pharmaceutical and biomedical analysis
VOLUME: 3
Page Numbers: 251-7
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ISSN: 0731-7085
DAY: 26
MONTH: 07
YEAR: 1985
Determination of cortisol and cortisone in urine using high-performance liquid chromatography with UV detection. Information
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LANGUAGE: eng
NlmUniqueID: 8309336
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Grant and Affiliation Information for Determination of cortisol and cortisone in urine using high-performance liquid chromatography with UV detection.
AFFILIATION: University of Oxford, Department of Biological Anthropology, 58 Banbury Road, Oxford OX2 6QS, UK.
Country: England
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MEDLINETA: J Pharm Biomed Anal
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