[Determination of chlorhexidine acetate in chlorhexidine acetate suppositories by high performance liquid chromatography coupled with hollow fiber membrane solvent microextraction]

[Determination of chlorhexidine acetate in chlorhexidine acetate suppositories by high performance liquid chromatography coupled with hollow fiber membrane solvent microextraction] Research Abstract Details 

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[Determination of chlorhexidine acetate in chlorhexidine acetate suppositories by high performance liquid chromatography coupled with hollow fiber membrane solvent microextraction] Abstract Text:

Huafeng Fu,Jiyu Guan,Zhishuang Qu,Jianmin Bao,

A novel method was developed to determine the content of chlorhexidine acetate (CA) in chlorhexidine acetate suppositories by high performance liquid chromatography (HPLC) coupled with hollow fiber membrane solvent microextraction. The sample was extracted with 1.5 mol/L acetic acid solution five times, then a hollow fiber containing n-octanol was placed in the sample solution to perform microextraction for extracting 20 min. The extract was analyzed by HPLC with UV detection at 260 nm. The chromatographic conditions were as following: acetonitrile-redistilled water (29:71, v/v, containing 0.3% triethylamine, pH 3.0) as mobile phase with the flow rate of 1 mL/min. The calibration curve of CA was linear from 0.5 to 16 mg/L. The method recovery was over 98.0% with relative standard deviation less than 4.0%. By using hollow fiber membrane solvent microextraction, the enrichment of 24-fold was achieved. The method is specific, simple, fast, sensitive and suitable for the determination of chlorhexidine acetate.

[Determination of chlorhexidine acetate in chlorhexidine acetate suppositories by high performance liquid chromatography coupled with hollow fiber membrane solvent microextraction] Publishing Authors By Initials

H Fu,J Guan,Z Qu,J Bao,

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[Determination of chlorhexidine acetate in chlorhexidine acetate suppositories by high performance liquid chromatography coupled with hollow fiber membrane solvent microextraction] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Se pu = Chinese journal of chromatography / Zhongg

VOLUME: 24

Page Numbers: 566-9

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ISSN: 1000-8713

DAY: 9

MONTH: Nov

YEAR: 2006

[Determination of chlorhexidine acetate in chlorhexidine acetate suppositories by high performance liquid chromatography coupled with hollow fiber membrane solvent microextraction] Information

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LANGUAGE: chi

NlmUniqueID: 9424804

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Grant and Affiliation Information for [Determination of chlorhexidine acetate in chlorhexidine acetate suppositories by high performance liquid chromatography coupled with hollow fiber membrane solvent microextraction]

AFFILIATION: College of Pharmaceutical Science and Biotechnology, Tianjin University, Tianjin 300072, China. fuhuafeng_tju@yahoo.com.cn

Country: China

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MEDLINETA: Se Pu

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