Determination of caderofloxacin lactate in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studies.

Determination of caderofloxacin lactate in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studies. Research Abstract Details 

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Determination of caderofloxacin lactate in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studies. Abstract Text:

Li Liu,Xiaoyan Pang,Dongmei Zhang,Xiao Xu,Hanyan Liu,Yan Liang,Lin Xie,Xiaodong Liu,Li Liu,Xiaoyan Pang,Dongmei Zhang,Xiao Xu,Hanyan Liu,Yan Liang,Lin Xie,Xiaodong Liu,Li Liu,Xiaoyan Pang,Dongmei Zhang,Xiao Xu,Hanyan Liu,Yan Liang,Lin Xie,Xiaodong Liu,

A sensitive liquid chromatography-electrospray ionization mass spectrometric (LC-ESI-MS) method for the quantification of a newly active quinolone carboxylic acid caderofloxacin lactate in rat plasma was developed and validated after precipitation method with methanol. Chromatographic separation was achieved on a reversed-phase Shimadzu 2.0mum C18 column (150mmx2.00mm) with the mobile phase of methanol-0.02% formic acid and step gradient elution resulted in a total run time of about 10.0min. The analytes were detected by using an electrospray positive ionization mass spectrometry in the selected ion monitoring (SIM) mode. A good linear relationship was obtained in the concentration range studied (5-2000ng/mL) (r=0.9998). The lowest limit of quantification (LLOQ) was 5ng/mL and the lowest limit of detection (LLOD) was 2ng/mL. Average recoveries ranged from 88.80 to 93.05% in plasma at the concentrations of 10, 100 and 1000ng/mL. Intra- and inter-day relative standard deviations were 4.01-7.30 and 4.15-7.51%, respectively. This method was successfully applied in the pharmacokinetic studies in rats.

Determination of caderofloxacin lactate in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studies. Publishing Authors By Initials

L Liu,X Pang,D Zhang,X Xu,H Liu,Y Liang,L Xie,X Liu,L Liu,X Pang,D Zhang,X Xu,H Liu,Y Liang,L Xie,X Liu,L Liu,X Pang,D Zhang,X Xu,H Liu,Y Liang,L Xie,X Liu,

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Determination of caderofloxacin lactate in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studies. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of pharmaceutical and biomedical analysis

VOLUME: 45

Page Numbers: 799-803

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ISSN: 0731-7085

DAY: 26

MONTH: 07

YEAR: 2007

Determination of caderofloxacin lactate in rat plasma by high-performance liquid chromatography-mass spectrometry and its application in rat pharmacokinetic studies. Information

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LANGUAGE: eng

NlmUniqueID: 8309336

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AFFILIATION: Key laboratory of Drug Metabolism and Pharmacokinetics, 24 Tong Jia Xiang Street, Mail Box 210, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China.

Country: England

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MEDLINETA: J Pharm Biomed Anal

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