Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry.

Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry. Abstract Text:

J Lee,H Kim,J C Jeong,E S Park,K W Hwang,S J Yang,J H Jeong,J Lee,H Kim,J C Jeong,E S Park,K W Hwang,S J Yang,J H Jeong,J Lee,H Kim,J C Jeong,E S Park,K W Hwang,S J Yang,J H Jeong,

A simple, rapid, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of beraprost, a stable, orally active prostacyclin analogue with vasodilatory, antiplatelet and cytoprotective effects. The analyte and internal standard, indomethacin, were extracted by solid-phase extraction using OASIS HLB cartridge. The chromatographic separation was performed on a C(18) column with a mobile of 0.1% formic acid-methanol (30:70, v/v). The highest daughter ion of deprotonated analyte was quantitated in negative ionization by multiple reactions monitoring with a mass spectrometer. The mass transitions m/z 397>269 and m/z 356>312 were used to measure beraprost and internal standard, respectively. The assay exhibited a linear range from 0.02 to 2ng/mL for beraprost in human plasma. The lower limit of quantitation was 20pg/mL with a relative standard deviation of less than 20%. The method was validated with respect to linearity, sensitivity, specificity, recovery, accuracy and precision. The validated method has been successfully used to analyze human plasma samples for application in pharmacokinetic study.

Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry. Publishing Authors By Initials

J Lee,H Kim,JC Jeong,ES Park,KW Hwang,SJ Yang,JH Jeong,J Lee,H Kim,JC Jeong,ES Park,KW Hwang,SJ Yang,JH Jeong,J Lee,H Kim,JC Jeong,ES Park,KW Hwang,SJ Yang,JH Jeong,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of chromatography. B, Analytical technolog

VOLUME: 859

Page Numbers: 229-33

Journal Abbreviation:

ISSN: 1570-0232

DAY: 7

MONTH: 10

YEAR: 2007

Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101139554

Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry.

AFFILIATION: College of Pharmacy, Kangwon National University, Chuncheon, Republic of Korea; Department of Drug Development Supporting Division, Seoul Pharma Laboratories Co. Ltd. (SPL), Seoul, Republic of Korea.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Chromatogr B Analyt Technol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry Related Publications

• Alternative and improved syntheses of highly potent and selective A3 adenosine receptor agonists, Cl-IB-MECA and thio-Cl-IB-MECA.
• Anti-inflammatory mechanisms of apigenin: inhibition of cyclooxygenase-2 expression, adhesion of monocytes to human umbilical vein endothelial cells, and expression of cellular adhesion molecules.
• Characterization of emissions composition for selected household products available in Korea.
• Computerized assessment of cognitive impairment in narcoleptic patients.
• Design, synthesis, and molecular modeling studies of 5'-deoxy-5'-ureidoadenosine: 5'-ureido group as multiple hydrogen bonding donor in the active site of S-adenosylhomocysteine hydrolase.
• Determination of beraprost in human plasma by a high-performance liquid chromatography-tandem mass spectrometry.
• Effects of diarylheptanoids on the tumor necrosis factor-alpha-induced expression of adhesion molecules in human umbilical vein endothelial cells.
• Effects of news about genetics and obesity on controllability attribution and helping behavior.
• Expression of G1 cell cycle regulators in rat liver upon repeated exposure to thioacetamide.
• Generation of a Mig-6 conditional null allele.
• Hepatoprotective and antioxidant effects of the coffee diterpenes kahweol and cafestol on carbon tetrachloride-induced liver damage in mice.
• Inflammatory mediators induced by intratracheal instillation of ultrafine amorphous silica particles.
• Inhibition of the angiogenesis by the MCP-1 (monocyte chemoattractant protein-1) binding peptide.
• Multi-figure anatomical objects for shape statistics.
• Protective effects of puerarin on carbon tetrachloride-induced hepatotoxicity.
• Protective effects of vacuolar H+ -ATPase c on hydrogen peroxide-induced cell death in C6 glioma cells.
• Pulmonary nodular lymphoid hyperplasia associated with Sjögren's syndrome.
• Relationship of thyroid stimulating hormone with coronary atherosclerosis in angina patients.
• Secretory expression of active clostripain in Escherichia coli.
• Simple method for the temporal characterization of amplified spontaneous emission in femtosecond terawatt Ti:sapphire lasers.
• Standardized tests of heart rate variability for autonomic function tests in healthy koreans.
• Transverse subtrochanteric shortening osteotomy in primary total hip arthroplasty for patients with severe hip developmental dysplasia.
• Two distinct anion-binding modes and their relative stabilities.
• [A case of the treatment in an adult with hepatic undifferentiated (embryonal) sarcoma]
• [Docetaxel-cisplatin-5-FU combination chemotherapy as a first-line treatment in patients with metastatic or recurred gastric cancer]
• [Effects of a newborn care education program on newborn care confidence and behavioral accuracy of primiparas in a postpartum care center]
• [Endoscopic submucosal dissection for early gastric cancer and gastric adenoma]
• [PIVKA-II as a serological marker of hepatocellular carcinoma]
• [The effect of Tai Chi movement in patients with rheumatoid arthritis]
• [Treatment of chronic hepatitis C: shorter treatment duration for genotype 2 or 3 infection]