Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction.

Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction. Research Abstract Details 

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Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction. Abstract Text:

A Shahid,A A Siddiqui,T Sultana,H Qureshi,M A Waqar,S J Zuberi,

OBJECTIVE: To confirm alpha-1-AT deficiency status in duodenal ulcer using a combination of PCR and restricted enzyme digestion. METHODS: Fifty patients with endoscopically proven duodenal ulcer and hundred controls with no signs of the disease were included. Alpha-1-AT phenotypes were confirmed by polymerase chain reaction followed by restriction enzyme digestion. RESULTS: Alpha-1-AT concentration in duodenal ulcer patients showed a mean value of 2.12 +/- 0.11 g/l (range: 0.52-3.95 g/l, p < 0.05). In controls this was 2.47 +/- 0.08 g/l (range: 0.52-5.0 g/l). Among the controls 70% had the MM phenotype, 28% M1 M2 and 2% FM. In duodenal ulcer, MM predominates (70%), followed by M1 M2 (18%), SS (4%), SZ (4%), ZZ (2%) and MZ (2%). CONCLUSION: Alpha-1 AT deficiency was found in 10% of duodenal ulcer patients. DNA analysis more accurately resolved the phenotypes as S and Z mutations.

Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction. Publishing Authors By Initials

A Shahid,AA Siddiqui,T Sultana,H Qureshi,MA Waqar,SJ Zuberi,

For similar skin and connective tissue diseases: connective tissue diseases: alpha 1-antitrypsin deficiency research abstracts see: skin and connective tissue diseases: connective tissue diseases: alpha 1-antitrypsin deficiency research

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Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: JPMA. The Journal of the Pakistan Medical Associat

VOLUME: 52

Page Numbers: 545-8

Journal Abbreviation:

ISSN: 0030-9982

DAY: 12

MONTH: Dec

YEAR: 2002

Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction. Information

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LANGUAGE: eng

NlmUniqueID: 7501162

Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction. Keywords Mesh Terms:

KEYWORDS: alpha 1-Antitrypsin Deficiency

MESH TERMS: genetics

Chemical & Substance for Abstract: Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction. Information

Substance Name: alpha 1-Antitrypsin

Registry Number: 0

Grant and Affiliation Information for Determination of alpha-1 antitrypsin genetic deficiency in duodenal ulcer by polymerase chain reaction.

AFFILIATION: PMRC Research Centre, Jinnah Postgraduate Medical Centre, Karachi.

Country: Pakistan

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MEDLINETA: J Pak Med Assoc

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