Determination of adenosine effects and adenosine receptors in murine corpus cavernosum.

Determination of adenosine effects and adenosine receptors in murine corpus cavernosum. Research Abstract Details 

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Determination of adenosine effects and adenosine receptors in murine corpus cavernosum. Abstract Text:

Rita C Tostes,Fernanda R C Giachini,Fernando S Carneiro,Romulo Leite,Edward W Inscho,R Clinton Webb,

This study tested the hypothesis that adenosine, in murine corpora cavernosa, produces direct relaxation of smooth muscle cells and inhibition of contractile responses mediated by sympathetic nerve stimulation. Penes were excised from anesthetized male C57BL/6 mice, dissected, and cavernosal strips were mounted to record isometric force. Adenosine, 2-chloroadenosine (stable analog of adenosine), and 2-phenylaminoadenosine (CV1808) (A2(A)/A2(B) agonist) produced concentration-dependent relaxations of phenylephrine-contracted tissues. Relaxation to 2-chloroadenosine was inhibited, in a concentration-dependent manner, by 2-(2-furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][1,2,4]triazolo[1,5-c]pyrimidin-5-amine (SCH58261; A2(A) antagonist; 10(-9)-10(-6) M) and N-(4-acetylphenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1,3-dipropyl-1H-purin-8-yl)phenoxy]acetamida (MRS1706; A2(B) antagonist; 10(-8)-10(-6) M). The combination of both antagonists abrogated 2-chloroadenosine-induced relaxation. Electrical field stimulation (EFS; 1-32 Hz) of adrenergic nerves produced frequency-dependent contractions that were inhibited by compounds that increase adenosine levels, such as 5'-iodotubercidin (adenosine kinase inhibitor), erythro-9-(2-hydroxy-3-nonyl)adenine (adenosine deaminase inhibitor), and dipyridamole (inhibitor of adenosine transport). The adenosine A1 receptor agonist N(6)-cyclopentyladenosine (C8031) right-shifted contractile responses to EFS, with a significant inhibitory effect at 10(-6) M. Blockade of adenosine A1 receptors with 8-cyclopentyl-1,3-dipropylxanthine (C101) (10(-7) M) enhanced contractile responses to EFS and eliminated the inhibitory effects of 5'-iodotubercidin. Dipyridamole and 5'-iodotubercidin had no effect on adenosine-mediated relaxation. In summary, adenosine directly relaxes cavernosal smooth muscle cells, by the activation of A2(A)/A2(B) receptor subtypes. In addition, adenosine negatively modulates sympathetic neurotransmission, by A1 receptor subtype activation, in murine corpora cavernosa. Adenosine may subserve dual roles in modulating the physiological mechanisms of erection in mice.

Determination of adenosine effects and adenosine receptors in murine corpus cavernosum. Publishing Authors By Initials

RC Tostes,FR Giachini,FS Carneiro,R Leite,EW Inscho,RC Webb,

For similar heterocyclic compounds: heterocyclic compounds, 1-ring: azoles: triazoles research abstracts see: heterocyclic compounds: heterocyclic compounds, 1-ring: azoles: triazoles research

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MEDLINE DATE:

Determination of adenosine effects and adenosine receptors in murine corpus cavernosum. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of pharmacology and experimental thera

VOLUME: 322

Page Numbers: 678-85

Journal Abbreviation: J. Pharmacol. Exp. Ther.

ISSN: 0022-3565

DAY: 9

MONTH: 05

YEAR: 2007

Determination of adenosine effects and adenosine receptors in murine corpus cavernosum. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376362

Determination of adenosine effects and adenosine receptors in murine corpus cavernosum. Keywords Mesh Terms:

KEYWORDS: Triazoles

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Determination of adenosine effects and adenosine receptors in murine corpus cavernosum. Information

Substance Name: terazosine

Registry Number: 63590-64-7

Grant and Affiliation Information for Determination of adenosine effects and adenosine receptors in murine corpus cavernosum.

AFFILIATION: Department of Pharmacology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil. rtostes@mcg.edu

Country: United States

AGENCY: United States NHLBI

GRANT: HL74167

ACRONYM: HL

MEDLINETA: J Pharmacol Exp Ther

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