Determination and pharmacokinetic study of indirubin in rat plasma by high-performance liquid chromatography.

Determination and pharmacokinetic study of indirubin in rat plasma by high-performance liquid chromatography. Research Abstract Details 

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Determination and pharmacokinetic study of indirubin in rat plasma by high-performance liquid chromatography. Abstract Text:

X Y Deng,S N Zheng,G H Gao,G Fan,F Li,

A specific, simple and sensitive HPLC method with UV detection was developed and validated for the pharmacokinetic studies of indirubin in rat plasma for the first time. Indirubin, with osthole as the internal standard, was extracted from plasma samples by liquid-liquid extraction. Chromatographic separation was conducted on a reverse-phase ODS column (200mmx4.6mm, i.d., 5mum), using a mixture of methanol-water (75:25, v/v) as the mobile phase at a flow rate of 1.0ml/min with UV detection at 289nm. The calibration curve of indirubin was linear over the range of 6.5-1950ng/ml in rat plasma. The lower limit of quantification (LLOQ) was found to be 6.5ng/ml. The present method was successfully applied for estimating the pharmacokinetic parameters of indirubin following intravenous and intraperitoneal administration of indirubin to rats.

Determination and pharmacokinetic study of indirubin in rat plasma by high-performance liquid chromatography. Publishing Authors By Initials

XY Deng,SN Zheng,GH Gao,G Fan,F Li,

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Determination and pharmacokinetic study of indirubin in rat plasma by high-performance liquid chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Phytomedicine : international journal of phytother

VOLUME: 15

Page Numbers: 277-83

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ISSN: 1618-095X

DAY: 11

MONTH: 03

YEAR: 2008

Determination and pharmacokinetic study of indirubin in rat plasma by high-performance liquid chromatography. Information

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LANGUAGE: eng

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Country: Germany

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MEDLINETA: Phytomedicine

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