Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis.

Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis. Research Abstract Details 

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Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis. Abstract Text:

Fumiko Nishikawa,Kazuyoshi Murakami,Ken Noda,Takashi Yokoyama,Satoshi Nishikawa,

We performed in vitro selection against bovine prion protein (bPrP) using RNA pools with thirty-nucleotides randomized regions. Among the obtained RNA aptamers, RNA aptamer wt2 possessed four GGA triplets. It is known that RNA and DNA oligomers containing GGA repeat sequences show a G-quadruplex structure in the presence of K+ or Na+ ions using the NMR and crystallographic studies. Recently we have been studying RNA aptamer-protein, -peptide and -RNA interactions using microchip electrophoresis (ME). In this study, we used this system to detect structural changes of RNA aptamer wt2 in the presence of K+ and Na+ ions, and detected the dimer formation with simple operation.

Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis. Publishing Authors By Initials

F Nishikawa,K Murakami,K Noda,T Yokoyama,S Nishikawa,

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Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nucleic acids symposium series (2004)

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Page Numbers: 397-8

Journal Abbreviation: Nucleic Acids Symp Ser (Oxf)

ISSN: 1746-8272

DAY: 21

MONTH: 11

YEAR: 2007

Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Detection of structural changes of RNA aptamer containing GGA repeats under the ionic condition using the microchip electrophoresis.

AFFILIATION: Age Dimension Research Center, AIST, Tsukuba, Japan.

Country: England

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MEDLINETA: Nucleic Acids Symp Ser (Oxf)

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