Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid.

Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. Research Abstract Details 

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Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. Abstract Text:

S C Pflugfelder,Z Liu,D Monroy,D Q Li,M E Carvajal,S A Price-Schiavi,N Idris,A Solomon,A Perez,K L Carraway,

PURPOSE: To evaluate human ocular surface epithelium and tear fluid for the presence of sialomucin complex (MUC4), a high-molecular-weight heterodimeric glycoprotein composed of mucin (ASGP-1) and transmembrane (ASGP-2) subunits. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot analysis assays were used to identify sialomucin complex RNA in ocular surface epithelia. Immunoprecipitation and immunoblot analysis were used to identify immunoreactive species in human tears and in the corneal and conjunctival epithelia using antibodies specific for carbohydrate and peptide epitopes on the sialomucin complex subunits. Immunofluorescence staining was used to detect sialomucin complex in frozen sections and impression cytology specimens of human cornea and conjunctival epithelia. RESULTS: ASGP-1- and ASGP-2-specific sequences were amplified from RNA extracted from both conjunctival and corneal epithelial biopsies by RT-PCR. Sialomucin complex transcripts were also detected in these tissues by Northern blot analysis, with a greater level of RNA detected in the peripheral than the central corneal epithelium. Sialomucin complex was immunoprecipitated from tear fluid samples and both corneal and conjunctival epithelia and detected by immunoblot analysis with specific anti-ASGP-1 and anti-ASGP-2 antibodies. The ASGP-1 peptide antibody HA-1 stained the full thickness of the corneal and conjunctival epithelia. In contrast, antibody 15H10, which reacts against a carbohydrate epitope on ASGP-1, stained only the superficial epithelial layers of these tissues. No staining was observed in the conjunctival goblet cells. CONCLUSIONS: Sialomucin complex was originally identified in rat mammary adenocarcinoma cells and has recently been shown to be produced by the ocular surface epithelia of rats. Furthermore, it has been identified as the rat homologue of human MUC4 mucin. The present studies show that it is expressed in the stratified epithelium covering the surface of the human eye and is present in human tear fluid. Expression of a carbohydrate-dependent epitope on the mucin subunit (ASGP-1) of sialomucin complex occurs in a differentiation-dependent fashion. Sialomucin complex joins MUC1 as another membrane mucin produced by the human ocular surface epithelia but is also found in the tear fluid, presumably in a soluble form, as found on the rat ocular surface.

Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. Publishing Authors By Initials

SC Pflugfelder,Z Liu,D Monroy,DQ Li,ME Carvajal,SA Price-Schiavi,N Idris,A Solomon,A Perez,KL Carraway,

For similar fluids and secretions: bodily secretions: tears research abstracts see: fluids and secretions: bodily secretions: tears research

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Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Investigative ophthalmology & visual science

VOLUME: 41

Page Numbers: 1316-26

Journal Abbreviation: Invest. Ophthalmol. Vis. Sci.

ISSN: 0146-0404

DAY: 1

MONTH: May

YEAR: 2000

Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. Information

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LANGUAGE: eng

NlmUniqueID: 7703701

Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. Keywords Mesh Terms:

KEYWORDS: Tears

MESH TERMS: chemistry

Chemical & Substance for Abstract: Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Detection of sialomucin complex (MUC4) in human ocular surface epithelium and tear fluid.

AFFILIATION: Ocular Surface and Tear Center, Bascom Palmer Eye Institute, University of Miami School of Medicine, Florida 33136, USA. spflugfelder@bpei.med.miami.edu

Country: UNITED STATES

AGENCY: United States NEI

GRANT: EY12343

ACRONYM: EY

MEDLINETA: Invest Ophthalmol Vis Sci

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