Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis.

Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis. Research Abstract Details 

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Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis. Abstract Text:

D Flemming Hansen,Mathias A S Hass,Hans M Christensen,Jens Ulstrup,Jens J Led,

An NMR approach is presented that provides detailed information about short-lived, transient interactions between protein molecules in solution. The approach is based on the longitudinal paramagnetic relaxation rates of the protein nuclei and requires that at least one of the interacting molecules is paramagnetic. The specific interactions are monitored by the intermolecular paramagnetic contribution to the relaxation of protons at or close to the interaction surface. By applying the approach to plastocyanin from Anabaena variabilis, specific regions of interaction that may be involved in the electron self-exchange process of this plastocyanin were identified. This is in accord with recent 15N NMR relaxation studies of the backbone dynamics of Anabaena variabilis plastocyanin, with site-directed mutagenesis studies of the functional importance of the corresponding regions in Phormidium laminosum plastocyanin and with the crystal packing surface of P. laminosum plastocyanin.

Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis. Publishing Authors By Initials

DF Hansen,MA Hass,HM Christensen,J Ulstrup,JJ Led,

For similar proteins: metalloproteins: plastocyanin research abstracts see: proteins: metalloproteins: plastocyanin research

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Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 6858-9

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 11

MONTH: Jun

YEAR: 2003

Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis. Keywords Mesh Terms:

KEYWORDS: Plastocyanin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis. Information

Substance Name: Plastocyanin

Registry Number: 9014-09-9

Grant and Affiliation Information for Detection of short-lived transient protein-protein interactions by intermolecular nuclear paramagnetic relaxation: plastocyanin from Anabaena variabilis.

AFFILIATION: Department of Chemistry, University of Copenhagen, Universitetsparken 5, DK-2100 Copenhagen Ø, Denmark.

Country: United States

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MEDLINETA: J Am Chem Soc

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