Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria.

Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Research Abstract Details 

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Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Abstract Text:

Shannon R Mackey,Jayna L Ditty,Eugenia M Clerico,Susan S Golden,

The unicellular cyanobacterium Synechococcus elongatus PCC 7942 is the model organism for studying prokaryotic circadian rhythms. Although S. elongatus does not display an easily measurable overt circadian behavior, its gene expression is under circadian control; hence, a "behavior" is created by linking a cyanobacterial promoter to either the bacterial luxAB or firefly luc luciferase genes to create reporter fusions whose activity can be easily monitored by bioluminescence. Numerous vectors have been created in our lab for introducing luciferase reporter genes into the S. elongatus chromosome. A choice of methods and equipment to detect light production from the luciferase fusions provides a means for high-throughput, automated mutant screens as well as testing rhythms from two promoter fusions within the same cell culture.

Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Publishing Authors By Initials

SR Mackey,JL Ditty,EM Clerico,SS Golden,

For similar genetic processes: recombination, genetic: transformation, genetic research abstracts see: genetic processes: recombination, genetic: transformation, genetic research

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MEDLINE DATE:

Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 362

Page Numbers: 115-29

Journal Abbreviation:

ISSN: 1064-3745

DAY: 3

MONTH: 12

YEAR: 2007

Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9214969

Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Keywords Mesh Terms:

KEYWORDS: Transformation, Genetic

MESH TERMS: physiology

Chemical & Substance for Abstract: Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Information

Substance Name: Luciferases, Firefly

Registry Number: EC 1.13.12.7

Grant and Affiliation Information for Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria.

AFFILIATION: Department of Biology, Texas A&M University, College Station, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM62419

ACRONYM: GM

MEDLINETA: Methods Mol Biol

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