Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep.

Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep. Research Abstract Details 

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Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep. Abstract Text:

J A Navarro,J Sanchez,A Bernabe,M A Gomez,S Gomez,J Seva,

The anti-Mycobacterium paratuberculosis polyclonal serum is proved useful for labelling Mycobacterium paratuberculosis in glutaraldehyde-osmium-fixed and epon-embedded intestinal samples from sheep with clinical symptoms of paratuberculosis. M. paratuberculosis marked with antibody-coated colloidal gold stain was seen in macrophages, epithelioid cells, giant cells and neutrophils throughout intestinal mucosa. In large macrophages with a low lysosomal content, a great number of intact mycobacteria was seen within phagosomes. In macrophages with average lysosomal content, very few intact mycobacteria or mycobacterial debris were present and lysosome-phagosome fusions were observed. Mycobacteria within neutrophils were scanty. These results show the usefulness of colloidal immunogold techniques for studies of the pathogenesis of paratuberculosis.

Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep. Publishing Authors By Initials

JA Navarro,J Sanchez,A Bernabe,MA Gomez,S Gomez,J Seva,

For similar animal diseases: sheep diseases research abstracts see: animal diseases: sheep diseases research

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Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zentralblatt für Veterinärmedizin. Reihe B. Journa

VOLUME: 39

Page Numbers: 421-7

Journal Abbreviation: Zentralblatt Veterinarmedizin

ISSN: 0514-7166

DAY: 13

MONTH: Aug

YEAR: 1992

Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep. Information

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LANGUAGE: eng

NlmUniqueID: 331325

Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep. Keywords Mesh Terms:

KEYWORDS: Sheep Diseases

MESH TERMS: microbiology

Chemical & Substance for Abstract: Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep. Information

Substance Name: Antigens, Bacterial

Registry Number: 0

Grant and Affiliation Information for Detection of Mycobacterium paratuberculosis antigen with colloidal immunogold in naturally infected sheep.

AFFILIATION: Unidad Docente de Histología y Anatomía Patológica, Facultad de Veterinaria, Universidad de Murcia, España.

Country: GERMANY

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MEDLINETA: Zentralbl Veterinarmed B

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