Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications.

Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications. Research Abstract Details 

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Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications. Abstract Text:

Kazuhiko Mori,Tomohiro Suzuki,Hiroshi Uozaki,Hayao Nakanishi,Tetsuya Ueda,Yoshihiro Matsuno,Yasuhiro Kodera,Hiromi Sakamoto,Nobuko Yamamoto,Mitsuru Sasako,Michio Kaminishi,Hiroki Sasaki,

BACKGROUND: Peritoneal cytology is an important prognostic factor of gastric cancer. However, peritoneal cytology requires great skill, which may explain its low prevalence. A reverse transcriptase-polymerase chain reaction-based assay with multiple marker genes or immunocytochemistry was assessed as an alternative method of gathering the same kind of data as cytology. METHODS: Peritoneal washings from 179 patients with gastric cancer were analyzed by multiplex reverse transcriptase-polymerase chain reaction with 10 marker genes and subsequent hybridization to a customized oligo-nucleotide array. Results with this assay were either validated as a prognostic factor or confirmed by demonstrating the presence of cancer cells by immunocytochemical cytology. RESULTS: Only 1 (2.2%) of 44 disease-free cases was shown to be positive by the microarray assay, whereas 13 (93%) of 14 conventional cytology-positive cases were found to be positive. This assay further detected approximately one-third of cytology-negative patients either with peritoneal recurrence (7 of 20, 35%) or with non-peritoneal recurrence (6 of 22, 27%). A high concordance between the microarray assay and immunocytochemical cytology with five antibodies against CK20, FABP1, MUC2, TFF1, and MASPIN was confirmed. The clinical outcome of the microarray assay-positive cases was poor, as was that of the cytology-positive cases. CONCLUSIONS: Our assay, though time-consuming and requiring special equipment, demonstrated a specificity and sensitivity equal to or better than cytology in our institutes. The minimal free peritoneal cancer cells detected by the microarray assay may provide the same clinical information as larger amounts of cancer cells for patients with gastric cancer. An anti-MASPIN antibody may be helpful in peritoneal cytology of gastric cancer.

Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications. Publishing Authors By Initials

K Mori,T Suzuki,H Uozaki,H Nakanishi,T Ueda,Y Matsuno,Y Kodera,H Sakamoto,N Yamamoto,M Sasako,M Kaminishi,H Sasaki,

For similar proteins: neoplasm proteins: tumor suppressor proteins research abstracts see: proteins: neoplasm proteins: tumor suppressor proteins research

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Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Annals of surgical oncology

VOLUME: 14

Page Numbers: 1694-702

Journal Abbreviation: Ann. Surg. Oncol.

ISSN: 1068-9265

DAY: 9

MONTH: 02

YEAR: 2007

Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications. Information

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LANGUAGE: eng

NlmUniqueID: 9420840

Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications. Keywords Mesh Terms:

KEYWORDS: Tumor Suppressor Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications. Information

Substance Name: Tumor Suppressor Proteins

Registry Number: 0

Grant and Affiliation Information for Detection of minimal gastric cancer cells in peritoneal washings by focused microarray analysis with multiple markers: clinical implications.

AFFILIATION: Department of Gastrointestinal Surgery, University of Tokyo Graduate School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo, 113-8655, Japan.

Country: United States

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MEDLINETA: Ann Surg Oncol

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