Detection of human alpha-herpesvirus DNA using consensus primers and specific probes.

Detection of human alpha-herpesvirus DNA using consensus primers and specific probes. Research Abstract Details 

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Detection of human alpha-herpesvirus DNA using consensus primers and specific probes. Abstract Text:

T Aono,S Murakami,N Yanagihara,K Yamanishi,

A simple and rapid diagnostic assay system was developed for detecting and identifying human alpha-herpesviruses, such as herpes simplex virus types 1 and 2 and varicella-zoster virus, by polymerase chain reaction. This system was based on an amplification step, using primers that bind the DNA polymerase consensus sequence of alpha-human herpesviruses, and a detection step, using non-radioactive virus-specific probes. This method could be used to amplify any human alpha-herpesviruses, and each virus-specific probe was highly specific for identification of the amplified product. This system is readily applicable for implementation in the clinical laboratory.

Detection of human alpha-herpesvirus DNA using consensus primers and specific probes. Publishing Authors By Initials

T Aono,S Murakami,N Yanagihara,K Yamanishi,

For similar investigative techniques: genetic techniques: nucleic acid amplification techniques: polymerase chain reaction research abstracts see: investigative techniques: genetic techniques: nucleic acid amplification techniques: polymerase chain reaction research

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Detection of human alpha-herpesvirus DNA using consensus primers and specific probes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta oto-laryngologica. Supplementum

VOLUME: 514

Page Numbers: 132-4

Journal Abbreviation:

ISSN: 0365-5237

DAY: 13

MONTH: 02

YEAR: 1994

Detection of human alpha-herpesvirus DNA using consensus primers and specific probes. Information

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LANGUAGE: eng

NlmUniqueID: 370355

Detection of human alpha-herpesvirus DNA using consensus primers and specific probes. Keywords Mesh Terms:

KEYWORDS: Polymerase Chain Reaction

MESH TERMS: genetics

Chemical & Substance for Abstract: Detection of human alpha-herpesvirus DNA using consensus primers and specific probes. Information

Substance Name: DNA, Viral

Registry Number: 0

Grant and Affiliation Information for Detection of human alpha-herpesvirus DNA using consensus primers and specific probes.

AFFILIATION: Department of Virology, Osaka University, Japan.

Country: NORWAY

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MEDLINETA: Acta Otolaryngol Suppl

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