Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction.

Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction. Research Abstract Details 

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Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction. Abstract Text:

Jinhong Zeng,Qiong Liu,Huibi Xu,Hongmei Wang,Jun Wang,Ruxiu Cai,

This paper aims at investigating a new method for the detection of DNA with catalytic beacons based on peroxidase-oxidase (PO) oscillation and analytic pulse perturbation technique. Two DNAzymes were constructed by the binding of specific DNA sequence with hemin or by the hybridization of target DNA with the catalytic beacon. Both DNAzymes possessed peroxidase-like activity and perturb the PO oscillator reaction when they were added into the oscillation system. The period and amplitude of oscillation increased significantly by both DNAzymes, which implied the decrease in the average rate of consumption of oxygen in solution, i.e., the decrease of the average rate of NADH oxidation. The results provide a new sensitive method for DNA detection and molecular recognition.

Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction. Publishing Authors By Initials

J Zeng,Q Liu,H Xu,H Wang,J Wang,R Cai,

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Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Conference proceedings : ... Annual International

VOLUME: 5

Page Numbers: 4658-61

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ISSN: 1557-170X

DAY: 6

MONTH: 02

YEAR: 2005

Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction. Information

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LANGUAGE: eng

NlmUniqueID: 101243413

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Grant and Affiliation Information for Detection of DNA with Catalytic Beacons Based on Peroxidase-oxidase Oscillating Reaction.

AFFILIATION: Chemistry Department of Huazhong University of Science and Technology, Wuhan Hubei 430074, China (e-mail: Jintao032961@163.com).

Country: United States

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MEDLINETA: Conf Proc IEEE Eng Med Biol So

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