Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling.

Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling. Abstract Text:

D Theil,R Hoop,A J Herring,A Pospischil,

In situ hybridization, (ISH) using a digoxigenin-antisense RNA-probe to detect chlamydial rRNA was applied to post mortem tissue of birds. The technique was optimized and validated using tissue from experimentally-infected chicken embryos. Tissue sections were also tested by immunohistochemistry (peroxidase-antiperoxidase reaction, PAP) for the presence of chlamydial antigen using a genus specific monoclonal antibody. In the chicken embryo tissue, ISH and PAP were comparably sensitive and specific (100% and 100%, respectively). ISH and PAP in general were correlated to microscopic lesions. For further comparison, ISH with PAP was applied retrospectively to tissues of 82 birds from which Chlamydia had been isolated, or which were suggestive of chlamydiosis. Using in situ hybridization 47 of 82 birds were found to be positive, and as were 23 of 82 birds with PAP. None of the ISH-only positive cases were found to be strongly positive. On the other hand, cases which were found positive with the ISH were also positive with other methods (PAP and isolation of Chlamydiae from chicken embryos). There was no close correlation between the positive cells and histological lesions. In spite of the higher sensitivity and specificity of the ISH, this technique is not suitable for routine diagnostic investigations. ISH is expensive, laborious, and time consuming.

Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling. Publishing Authors By Initials

D Theil,R Hoop,AJ Herring,A Pospischil,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, bacterial research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, bacterial research

PUBMED ID PMID:

MEDLINE DATE:

Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Zentralblatt für Veterinärmedizin. Reihe B. Journa

VOLUME: 43

Page Numbers: 365-70

Journal Abbreviation: Zentralblatt Veterinarmedizin

ISSN: 0514-7166

DAY: 13

MONTH: Aug

YEAR: 1996

Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 331325

Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling. Keywords Mesh Terms:

KEYWORDS: RNA, Bacterial

MESH TERMS: analysis

Chemical & Substance for Abstract: Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling. Information

Substance Name: RNA, Bacterial

Registry Number: 0

Grant and Affiliation Information for Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling.

AFFILIATION: Institut für Veterinärpathologie, Zürich, Switzerland.

Country: GERMANY

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Zentralbl Veterinarmed B

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization A comparison between in situ hybridization and peroxidase-antiperoxidase labelling Related Publications

• Clinical and ultrasonographic findings, diagnosis and treatment of pyelonephritis in 17 cows.
• Detection of Chlamydia in formalin-fixed and paraffin-embedded avian tissue by in situ hybridization. A comparison between in situ hybridization and peroxidase-antiperoxidase labelling.
• Evidence for persistent Chlamydia pneumoniae infection of human coronary atheromas.
• Meningoencephalitis caused by IBR virus in calves in Argentina.
• Polymerase chain reaction (PCR) detection of porcine Chlamydia trachomatis and ruminant Chlamydia psittaci serovar 1 DNA in formalin-fixed intestinal specimens from swine.
• Retrospective study of myocardial canine parvovirus infection by in situ hybridization.
• [Comparative light and electron microscopic demonstration of virus-induced inclusion bodies]
• [Immunohistochemical determination of Chlamydia psittaci/pecorum and C.trachomatis in the piglet gut]
• [Infections with enteropathogenic Escherichia coli (EPEC) in cattle. 1. Comparison of different animal models and a cell culture system for the establishment of a detection system for "attaching and effacing" (AE) lesions]
• [Rhinosporidiosis in the horse. Histological and electron microscopic study of a case]
• [The infectious causes of abortion and stillbirth in swine in Switzerland]