Detection of Brugia parasite DNA in human blood by real-time PCR.

Detection of Brugia parasite DNA in human blood by real-time PCR. Research Abstract Details 

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Detection of Brugia parasite DNA in human blood by real-time PCR. Abstract Text:

Ramakrishna U Rao,Gary J Weil,Kerstin Fischer,Taniawati Supali,Peter Fischer,Ramakrishna U Rao,Gary J Weil,Kerstin Fischer,Taniawati Supali,Peter Fischer,

Brugian filariasis (caused by the nematodes Brugia malayi and B. timori) is an important cause of disability in Southeast Asia. Improved diagnostic tests are needed for filariasis elimination programs (to identify areas of endemicity and to monitor progress) and for diagnosis of the disease in infected individuals. We have developed and evaluated two real-time PCR assays for detecting Brugia DNA in human blood and compared the results of these assays to those of "gold standard" assays. One assay uses a TaqMan probe (TaqM) to amplifiy a 320-bp "HhaI repeat" DNA sequence. The other assay uses a minor groove binding probe (MGB) and modified nucleotides in primers (Eclipse MGB) to amplify a 120-bp fragment of the HhaI repeat. This assay detects 22 copies of the target sequence, and it is more sensitive than the TaqM assay. Both assays were evaluated with human blood samples from two different areas of endemicity. The MGB assay was as sensitive as membrane filtration and microscopy for the detection of B. malayi infection in 57 blood samples recovered at night from patients in Sulawesi, Indonesia. The MGB assay also detected parasite DNA in 17 of 31 (55%) of microfilaria-negative day blood samples from these subjects. This test was more sensitive than the conventional and the TaqM PCRs (and was almost as sensitive as night blood membrane filtration) for the detection of infection in 52 blood samples recovered at night from individuals in an area of B. timori endemicity on Alor Island, Indonesia, where microfilaria-positive individuals had low densities after mass treatment. Thus, the Eclipse MGB real-time PCR assay is a sensitive means of detecting Brugia parasite DNA in human blood.

Detection of Brugia parasite DNA in human blood by real-time PCR. Publishing Authors By Initials

RU Rao,GJ Weil,K Fischer,T Supali,P Fischer,RU Rao,GJ Weil,K Fischer,T Supali,P Fischer,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

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Detection of Brugia parasite DNA in human blood by real-time PCR. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of clinical microbiology

VOLUME: 44

Page Numbers: 3887-93

Journal Abbreviation: J. Clin. Microbiol.

ISSN: 0095-1137

DAY: 6

MONTH: 09

YEAR: 2006

Detection of Brugia parasite DNA in human blood by real-time PCR. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505564

Detection of Brugia parasite DNA in human blood by real-time PCR. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: methods

Chemical & Substance for Abstract: Detection of Brugia parasite DNA in human blood by real-time PCR. Information

Substance Name: DNA, Helminth

Registry Number: 0

Grant and Affiliation Information for Detection of Brugia parasite DNA in human blood by real-time PCR.

AFFILIATION: Department of Internal Medicine, Infectious Diseases Division, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8051, St. Louis, MO 63110, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI-35855

ACRONYM: AI

MEDLINETA: J Clin Microbiol

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