Detection of avian influenza virus using an interferometric biosensor.

Detection of avian influenza virus using an interferometric biosensor. Research Abstract Details 

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Detection of avian influenza virus using an interferometric biosensor. Abstract Text:

Jie Xu,David Suarez,David S Gottfried,Jie Xu,David Suarez,David S Gottfried,

An interferometric biosensor immunoassay for direct and label-less detection of avian influenza through whole virus capture on a planar optical waveguide is described. The assay response is based on index of refraction changes that occur upon binding of virus particles to unique antigen-specific (hemagglutinin) antibodies on the waveguide surface. Three virus subtypes (two H7 and one H8) in buffer solution were tested using both monoclonal and polyclonal capture antibodies. The real-time response of the antigen-antibody interaction was measured and was shown to be concentration-dependent, with detection limits as low as 0.0005 hemagglutination units per milliliter. A simple sandwich assay was shown to further increase the biosensor response.

Detection of avian influenza virus using an interferometric biosensor. Publishing Authors By Initials

J Xu,D Suarez,DS Gottfried,J Xu,D Suarez,DS Gottfried,

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Detection of avian influenza virus using an interferometric biosensor. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Analytical and bioanalytical chemistry

VOLUME: 389

Page Numbers: 1193-9

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ISSN: 1618-2642

DAY: 21

MONTH: 08

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 101134327

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AFFILIATION: Georgia Tech Research Institute, Georgia Institute of Technology, Atlanta, GA 30332-0801, USA.

Country: Germany

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MEDLINETA: Anal Bioanal Chem

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