Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients.

Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients. Research Abstract Details 

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Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients. Abstract Text:

Om Parkash,Ajay Kumar,Astha Nigam,Bhawneshwar K Girdhar,

The prevalence of IgG antibodies against Mycobacterium leprae recombinant culture filtrate protein-10 (rCFP-10) was investigated in serum samples from 56 leprosy patients, 15 tuberculosis (TB) patients, 14 other skin-diseased patients and 20 healthy subjects. On classifying the patients into bacterial index (BI)-positive and BI-negative groups, the assay showed 83.3 % (15/18) sensitivity for detection of BI-positive leprosy patients. On the other hand, the sensitivity for detection of BI-negative patients was 18.4 % (7/38). None of the 15 TB patients and 14 other skin-diseased patients was positive; however, only one out of 20 healthy individuals was positive, indicating that antibody response to culture filtrate protein-10 (CFP-10) was highly specific (98.0 %; 48/49). Statistically, the performance of the CFP-10-based assay was found to be comparable (P>0.05) with that of an anti-phenolic glycolipid-I (PGL-I) antibody-detecting assay. Thus, M. leprae CFP-10 is potentially a specific antigen for measuring antibody response in BI-positive leprosy patients. Being a secreted antigen, CFP-10 may act as a marker for the viability of M. leprae inside the host, and hence its serological potential is worth exploring for application in monitoring the response of patients with BI-positive leprosy (a highly infectious form) during the course of chemotherapy. When comparing the bacteriological and serological results, an agreement of 82.1 % showed that seropositivity to M. leprae CFP-10 corresponded well with bacteriological criteria. Hence, CFP-10 seems to be a suitable antigen for classification of leprosy patients into BI-positive and BI-negative groups.

Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients. Publishing Authors By Initials

O Parkash,A Kumar,A Nigam,BK Girdhar,

For similar investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research abstracts see: investigative techniques: epidemiologic methods: statistics as topic: sensitivity and specificity research

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Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of medical microbiology

VOLUME: 55

Page Numbers: 1337-41

Journal Abbreviation: J. Med. Microbiol.

ISSN: 0022-2615

DAY: 3

MONTH: Oct

YEAR: 2006

Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients. Information

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LANGUAGE: eng

NlmUniqueID: 224131

Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients. Keywords Mesh Terms:

KEYWORDS: Sensitivity and Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Detection of antibodies against Mycobacterium leprae culture filtrate protein-10 in leprosy patients.

AFFILIATION: National JALMA Institute for Leprosy and other Mycobacterial Diseases, TajGanj, Agra-1, India. om1234@gmail.com

Country: England

AGENCY: United States NIAID

GRANT: 01 AI 25469

ACRONYM: AI

MEDLINETA: J Med Microbiol

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