Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR.

Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Research Abstract Details 

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Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Abstract Text:

M Y Halami,H Nieper,H Müller,R Johne,

Circoviruses are the causative agents of acute and chronic diseases in several animal species. Clinical symptoms of circovirus infections range from depression and diarrhoea to immunosuppression and feather disorders in birds. Eleven different members of the genus Circovirus are known so far, which infect pigs and birds in a species-specific manner. Here, a nested PCR was developed for the detection of a broad range of different circoviruses in clinical samples. Using this assay, a novel circovirus was detected in mute swans (Cygnus olor) found dead in Germany in 2006. Sequence analysis of the swan circovirus (SwCV) genome, amplified by multiply primed rolling-circle amplification and PCR, indicates that SwCV is a distinct virus most closely related to the goose circovirus (73.2% genome sequence similarity). Sequence variations between SwCV genomes derived from two different individuals were high (15.5% divergence) and mainly confined to the capsid protein-encoding region. By PCR testing of 32 samples derived from swans found dead in two different regions of Germany, detection rates of 20.0 and 77.3% were determined, thus indicating a high incidence of SwCV infection. The clinical significance of SwCV infection, however, needs to be investigated further.

Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Publishing Authors By Initials

MY Halami,H Nieper,H Müller,R Johne,

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Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Virus research

VOLUME: 132

Page Numbers: 208-12

Journal Abbreviation: Virus Res.

ISSN: 0168-1702

DAY: 20

MONTH: 12

YEAR: 2007

Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Information

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LANGUAGE: eng

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AFFILIATION: Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany.

Country: Netherlands

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MEDLINETA: Virus Res

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