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Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle.

Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle. Research Abstract Details 

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  • Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle. Abstract Text:

    saki naganoSaki Nagano,makoto matsubayashiMakoto Matsubayashi,toshimasa kitaToshimasa Kita,tsuguto narushimaTsuguto Narushima,isao kimataIsao Kimata,motohiro isekiMotohiro Iseki,tomoya hajiriTomoya Hajiri,hiroyuki taniHiroyuki Tani,kazumi sasaiKazumi Sasai,eiichiroh babaEiichiroh Baba,saki naganoSaki Nagano,makoto matsubayashiMakoto Matsubayashi,toshimasa kitaToshimasa Kita,tsuguto narushimaTsuguto Narushima,isao kimataIsao Kimata,motohiro isekiMotohiro Iseki,tomoya hajiriTomoya Hajiri,hiroyuki taniHiroyuki Tani,kazumi sasaiKazumi Sasai,eiichiroh babaEiichiroh Baba,saki naganoSaki Nagano,makoto matsubayashiMakoto Matsubayashi,toshimasa kitaToshimasa Kita,tsuguto narushimaTsuguto Narushima,isao kimataIsao Kimata,motohiro isekiMotohiro Iseki,tomoya hajiriTomoya Hajiri,hiroyuki taniHiroyuki Tani,kazumi sasaiKazumi Sasai,eiichiroh babaEiichiroh Baba,

    Cryptosporidium oocysts were detected in the feces of cattle in Saga, Japan. Isolates were morphologically large. We attempted to identify the species or genotypes of the isolates by analyzing the partial sequences of the 18S rRNA and Cryptosporidium oocyst wall protein (COWP) genes, and measuring the infectivity in mice. The isolates showed 100% homology with Cryptosporidium andersoni in the COWP gene sequence and it could be transmitted to mice, but in the 18S rRNA gene, there was an additional signal in the ABI sequence chromatogram. To examine the additional signal, we analyzed both the 18S rRNA and the COWP gene sequences of a single oocyst passaged from mice using a modified multiplex PCR that was able to amplify both genes. As a result, it was revealed that two distinct genotypes (Types A and B) of a novel C. andersoni type existed in the 18S rRNA gene, whereas the COWP gene sequences of both oocysts were identical to C. andersoni. Although the sequence of the 18S rRNA gene of Type A was identical to that of C. andersoni, that of Type B had a thymine insertion and was not identical to any sequence registered with GenBank. Here we report that this is a new type of C. andersoni.

    Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle. Publishing Authors By Initials

    s naganoS Nagano,m matsubayashiM Matsubayashi,t kitaT Kita,t narushimaT Narushima,i kimataI Kimata,m isekiM Iseki,t hajiriT Hajiri,h taniH Tani,k sasaiK Sasai,e babaE Baba,s naganoS Nagano,m matsubayashiM Matsubayashi,t kitaT Kita,t narushimaT Narushima,i kimataI Kimata,m isekiM Iseki,t hajiriT Hajiri,h taniH Tani,k sasaiK Sasai,e babaE Baba,s naganoS Nagano,m matsubayashiM Matsubayashi,t kitaT Kita,t narushimaT Narushima,i kimataI Kimata,m isekiM Iseki,t hajiriT Hajiri,h taniH Tani,k sasaiK Sasai,e babaE Baba,

    For similar abstracts research abstracts see: abstracts research

    PUBMED ID PMID:

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    Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Veterinary parasitology

    VOLUME: 149

    Page Numbers: 213-8

    Journal Abbreviation: Vet. Parasitol.

    ISSN: 0304-4017

    DAY: 6

    MONTH: 09

    YEAR: 2007

    Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 7602745

    Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle. Keywords Mesh Terms:

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    Grant and Affiliation Information for Detection of a mixed infection of a novel Cryptosporidium andersoni and its subgenotype in Japanese cattle.

    AFFILIATION: Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka 599-8531, Japan.

    Country: Netherlands

    Netherlands Research PublicationNetherlands Research Publication

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    MEDLINETA: Vet Parasitol

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