[Detection of 8-hydroxyguanine in the DNA of the H2O2-treated HL-60 cells by GC/FID]

[Detection of 8-hydroxyguanine in the DNA of the H2O2-treated HL-60 cells by GC/FID] Research Abstract Details 

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[Detection of 8-hydroxyguanine in the DNA of the H2O2-treated HL-60 cells by GC/FID] Abstract Text:

H Zhang,Q Zhu,L Mo,H Zhuang,C Cai,

This paper describes the DNA damage by hydrogen peroxide in HL-60 cells and the detection of 8-hydroxyguanine produced. The number of cells was at least about 2 x 10(7)/sample. Treatment of HL-60 cells with 0.4 mmol/L hydrogen peroxide for 24 h would result in DNA damage. The DNA samples were isolated, then qualitatively and quantitatively analyzed before hydrolysis. The results of experiments showed that 100-300 microL 98% formic acid could hydrolyze about 0.12 mg DNA samples completely in 150 degrees C for 1 h. After lyophilizing the DNA samples for 6-8 h, one DNA sample was trimethylsilylated completely with 150 microL BSTFA in 150 degrees C for 1 h. The recovery of trimethylsilylated 8-hydroxyguanine was 81.70%. The trimethylsilylated 8-hydroxyguanine was identified by GC/FID and CGC/MS-SIM parallely.

[Detection of 8-hydroxyguanine in the DNA of the H2O2-treated HL-60 cells by GC/FID] Publishing Authors By Initials

H Zhang,Q Zhu,L Mo,H Zhuang,C Cai,

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[Detection of 8-hydroxyguanine in the DNA of the H2O2-treated HL-60 cells by GC/FID] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Se pu = Chinese journal of chromatography / Zhongg

VOLUME: 17

Page Numbers: 262-4

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ISSN: 1000-8713

DAY: 28

MONTH: May

YEAR: 1999

[Detection of 8-hydroxyguanine in the DNA of the H2O2-treated HL-60 cells by GC/FID] Information

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LANGUAGE: chi

NlmUniqueID: 9424804

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Grant and Affiliation Information for [Detection of 8-hydroxyguanine in the DNA of the H2O2-treated HL-60 cells by GC/FID]

AFFILIATION: Institute of Medical Biochemistry, Guangdong Medical College, Zhanjiang, 524023.

Country: China

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MEDLINETA: Se Pu

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