Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR.

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR. Research Abstract Details 

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Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR. Abstract Text:

Min Li,Patrick L Green,

HTLV-1 and HTLV-2 are highly related delta-retroviruses that infect and transform T-lymphocytes, but have distinct pathogenic properties. HTLV replication and survival requires the expression of multiple gene products from an unspliced and a series of highly related alternatively spliced mRNA species. To date, the comparative levels of all known HTLV-1 and HTLV-2 viral mRNAs in different transformed cell lines and at different stages of virus infection have not been assessed. In this study, we compiled a series of oligonucleotide primer pairs and probes to quantify both HTLV-1 and HTLV-2 mRNA species using real-time RT-PCR. The optimized reaction for detection of each mRNA had amplification efficiency greater than 90% with a linear range spanning 25-2.5 x 10(7) copies. The R(2)'s of all standard curves were greater than 0.97. Quantitation of HTLV mRNAs between different cell lines showed variability (gag/pol>or=tax/rex>env>or=accessory proteins), but the overall levels of each mRNA relative to each other within a cell line were similar. These results provide a method to quantify all specific mRNAs from both HTLV-1 and HTLV-2, which can be used to evaluate further viral gene expression and correlate transcript levels to key stages of the virus life cycle and ultimately, pathogenesis.

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR. Publishing Authors By Initials

M Li,PL Green,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of virological methods

VOLUME: 142

Page Numbers: 159-68

Journal Abbreviation:

ISSN: 0166-0934

DAY: 6

MONTH: 03

YEAR: 2007

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR. Information

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LANGUAGE: eng

NlmUniqueID: 8005839

Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR. Information

Substance Name: Viral Proteins

Registry Number: 0

Grant and Affiliation Information for Detection and quantitation of HTLV-1 and HTLV-2 mRNA species by real-time RT-PCR.

AFFILIATION: Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USA.

Country: Netherlands

AGENCY: United States NCI

GRANT: R01 CA077556-07

ACRONYM: CA

MEDLINETA: J Virol Methods

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