Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method.

Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method. Research Abstract Details 

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Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method. Abstract Text:

Yi-Wei Tang,Hatim T Allawi,Marlene DeLeon-Carnes,Haijing Li,Stephen P Day,D Scott Schmid,Yi-Wei Tang,Hatim T Allawi,Marlene DeLeon-Carnes,Haijing Li,Stephen P Day,D Scott Schmid,

We report the use of a prototype Invader Plus method (Third Wave Technologies, Inc., Madison, WI) for the qualitative detection of varicella-zoster virus (VZV) and differentiation of wild-type and Oka vaccine VZV. The analytical sensitivity of the VZV Invader Plus reagents is at 10 copies per reaction. A total of 174 skin and mucous swab specimens were used to validate the assay's performance. The sensitivity and specificity were 98.3% and 98.1%, respectively, in comparison to a PCR-EIA assay. A perfect 100% agreement was obtained when VZV wild-type and vaccine differentiation was performed on 54 VZV-positive swab specimens against an allele-specific FRET real-time assay. The Invader Plus method provides another reliable tool for qualitative detection of VZV and differentiation of wild-type and vaccine virus.

Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method. Publishing Authors By Initials

YW Tang,HT Allawi,M DeLeon-Carnes,H Li,SP Day,DS Schmid,YW Tang,HT Allawi,M DeLeon-Carnes,H Li,SP Day,DS Schmid,

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Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of clinical virology : the official public

VOLUME: 40

Page Numbers: 129-34

Journal Abbreviation: J. Clin. Virol.

ISSN: 1386-6532

DAY: 28

MONTH: 08

YEAR: 2007

Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Detection and differentiation of wild-type and vaccine mutant varicella-zoster viruses using an Invader Plus method.

AFFILIATION: Department of Medicine, Vanderbilt University School of Medicine, Nashville, TN 37232, USA. yiwei.tang@vanderbilt.edu

Country: Netherlands

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MEDLINETA: J Clin Virol

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