Detection and characterization of muscle-specific nuclear proteins.

Detection and characterization of muscle-specific nuclear proteins. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Detection and characterization of muscle-specific nuclear proteins. Abstract Text:

D Takai,M Kirinoki,J Miyazaki,T Hirabayashi,

To identify nuclear proteins related to muscle tissue specificity, we tried to prepare antibodies recognizing muscle-specific nuclear proteins. Taking advantage of the autoimmunity of some nuclear proteins, we prepared an antiserum against chick muscle nuclear proteins by injecting protein components of the nuclei isolated from chick breast muscles into breast muscle. Three proteins, named p30, p32, and p37, were detected with the antiserum in a two-dimensional SDS-PAGE pattern of the isolated nuclei. P30 and p32 were not detected in the nuclei of liver, brain, cardiac muscle, or slow type skeletal muscle (anterior latissimus dorsi). They were detected in those of fast type skeletal muscle (pectoralis major and semitendinosus) and smooth muscle (gizzard) at all developmental stages examined. On serial fractionation of muscle cell nuclei, they were detected in a fraction obtained after DNase I treatment of the sample, suggesting that the proteins weakly bind to chromatin. A homology search of amino acid sequences showed that there is no known protein similar to p32.

Detection and characterization of muscle-specific nuclear proteins. Publishing Authors By Initials

D Takai,M Kirinoki,J Miyazaki,T Hirabayashi,

For similar investigative techniques: clinical laboratory techniques: cytological techniques: histocytological preparation techniques: staining and labeling research abstracts see: investigative techniques: clinical laboratory techniques: cytological techniques: histocytological preparation techniques: staining and labeling research

PUBMED ID PMID:

MEDLINE DATE:

Detection and characterization of muscle-specific nuclear proteins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 115

Page Numbers: 219-23

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1994

Detection and characterization of muscle-specific nuclear proteins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Detection and characterization of muscle-specific nuclear proteins. Keywords Mesh Terms:

KEYWORDS: Staining and Labeling

MESH TERMS: immunology

Chemical & Substance for Abstract: Detection and characterization of muscle-specific nuclear proteins. Information

Substance Name: Peptide Fragments

Registry Number: 0

Grant and Affiliation Information for Detection and characterization of muscle-specific nuclear proteins.

AFFILIATION: Institute of Biological Science, University of Tsukuba, Ibaraki.

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Detection and characterization of muscle-specific nuclear proteins Related Publications

• A comparative study of tropomyosin from vertebrate ventricles.
• A novel strategy for mammalian cell surface glycome profiling using lectin microarray.
• Accumulation of tropomyosin in developing chicken gizzard smooth muscle.
• Application of lectin microarray to crude samples: differential glycan profiling of lec mutants.
• Cell-specific expression of neutral glycosphingolipids in vertebrate brain: immunochemical localization of 3-O-acetyl-sphingosine-series glycolipid(s) in myelin and oligodendrocytes.
• Comparison of time trends in liver cancer incidence (1973-1997) in East Asia, Europe and USA, from Cancer Incidence in Five Continents Vol. IV-VIII.
• Comparison of time trends in ovary cancer incidence (1973-1997) in East Asia, Europe, and the USA, from Cancer Incidence in Five Continents Vols IV VIII.
• Detection and characterization of muscle-specific nuclear proteins.
• Ease of use of the Airway Scope vs the Bullard laryngoscope: a manikin study.
• Frontal affinity chromatography: sugar-protein interactions.
• Galectins: a family of animal lectins that decipher glycocodes.
• GlideScope-assisted endotracheal tube exchange.
• GlideScope-assisted insertion of a transesophageal echocardiography probe.
• Identification and distribution of tropomyosin isoforms in chicken digestive canal.
• Involvement of Ca2+-dependent proteasome in the degradation of both cyclin B1 and Mos during spontaneous activation of matured rat oocytes.
• Isolation and localization from chicken gizzard of an inhibitory protein for Mg2+-activated skeletal muscle actomyosin ATPase.
• Live rats resulting from injection of oocytes with spermatozoa freeze-dried and stored for one year.
• Many isoforms of fast muscle troponin T from chicken legs.
• Medical rehabilitation of the patients with spinal cord injury caused by aortic aneurysm and its operation.
• Nasotracheal intubation with the aid of the Airway Scope.
• On the heterogeneity and organ specificity of chicken tropomyosins.
• Preparation and Biodegradation of Sugar-Containing Poly(vinyl acetate) Emulsions.
• Randomized controlled study of 3 different types of hemoclips for hemostasis of bleeding canine acute gastric ulcers.
• Regulation of troponin T gene expression in chicken fast skeletal muscle: involvement of an M-CAT-like element distinct from the standard M-CAT.
• The StyletScope(R) facilitates tracheal intubation with the GlideScope(R).
• The functional characteristics conserved in tropomyosins.
• Tissue specificity of tropomyosin from the crayfish, Cambarus clarki.
• Tropomyosin isoforms in developing chicken gizzard smooth muscle.
• Troponin C-like protein in chicken gizzard muscle.
• Vasa vasorum and vascular lesions in the human abdominal aorta.