Detecting SNPs using a synthetic nanopore.

Detecting SNPs using a synthetic nanopore. Research Abstract Details 

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Detecting SNPs using a synthetic nanopore. Abstract Text:

Q Zhao,G Sigalov,V Dimitrov,B Dorvel,U Mirsaidov,S Sligar,A Aksimentiev,G Timp,

We have discovered a voltage threshold for permeation through a synthetic nanopore of dsDNA bound to a restriction enzyme that depends on the sequence. Molecular dynamic simulations reveal that the threshold is associated with a nanonewton force required to rupture the DNA-protein complex. A single mutation in the recognition site for the restriction enzyme, i.e., a single nucleotide polymorphism (SNP), can easily be detected as a change in the threshold voltage. Consequently, by measuring the threshold voltage in a synthetic nanopore, it may be possible to discriminate between two variants of the same gene (alleles) that differ in one base.

Detecting SNPs using a synthetic nanopore. Publishing Authors By Initials

Q Zhao,G Sigalov,V Dimitrov,B Dorvel,U Mirsaidov,S Sligar,A Aksimentiev,G Timp,

For similar natural sciences: chemistry: chemistry, physical: surface properties research abstracts see: natural sciences: chemistry: chemistry, physical: surface properties research

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Detecting SNPs using a synthetic nanopore. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Nano letters

VOLUME: 7

Page Numbers: 1680-5

Journal Abbreviation: Nano Lett.

ISSN: 1530-6984

DAY: 15

MONTH: 05

YEAR: 2007

Detecting SNPs using a synthetic nanopore. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101088070

Detecting SNPs using a synthetic nanopore. Keywords Mesh Terms:

KEYWORDS: Surface Properties

MESH TERMS: methods

Chemical & Substance for Abstract: Detecting SNPs using a synthetic nanopore. Information

Substance Name: Deoxyribonuclease EcoRI

Registry Number: EC 3.1.21.-

Grant and Affiliation Information for Detecting SNPs using a synthetic nanopore.

AFFILIATION: Beckman Institute, University of Illinois, Urbana, Illinois 61801, USA.

Country: United States

AGENCY: United States NHGRI

GRANT: R01 HG003713A

ACRONYM: HG

MEDLINETA: Nano Lett

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