Detailed methylation patterns and protein expression profiles of MGMT in colorectal carcinoma surgical margins.

Detailed methylation patterns and protein expression profiles of MGMT in colorectal carcinoma surgical margins. Research Abstract Details 

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Detailed methylation patterns and protein expression profiles of MGMT in colorectal carcinoma surgical margins. Abstract Text:

Dingdong Zhang,Yunfei Bai,Yan Wang,Junfeng Luo,Qinyu Ge,Yingjuan Qiao,Chao Jia,Zuhong Lu,Dingdong Zhang,Yunfei Bai,Yan Wang,Junfeng Luo,Qinyu Ge,Yingjuan Qiao,Chao Jia,Zuhong Lu,

OBJECTIVES:: Molecular margin analysis is considered more sensitive in detecting preneoplastic lesions and residual cancer cells than conventional histological margin examination. Hence, we examined MGMT expression profile and methylation status in histologically negative margins of colorectal cancer patients. DESIGN AND METHODS:: This study included 24 colorectal tumor tissues and corresponding negative surgical margin tissues. MGMT promoter methylation patterns were analyzed by using methylation-specific oligonucleotide microarray. In addition, MGMT protein expression was analyzed by immunohistochemistry. MGMT clinical significance was evaluated together with other well-known clinicopathological factors. RESULTS:: Extensive MGMT promoter methylation was observed in tumor tissues; a moderate methylation level was found in surgical margin tissues and little or no methylation was observed in the normal control. There was a trend towards longer overall survival for those patients with negative MGMT immunostaining in surgical margins. CONCLUSIONS:: MGMT expression negative in surgical margin tissues indicates longer overall survival for colorectal tumor patients.

Detailed methylation patterns and protein expression profiles of MGMT in colorectal carcinoma surgical margins. Publishing Authors By Initials

D Zhang,Y Bai,Y Wang,J Luo,Q Ge,Y Qiao,C Jia,Z Lu,D Zhang,Y Bai,Y Wang,J Luo,Q Ge,Y Qiao,C Jia,Z Lu,

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Detailed methylation patterns and protein expression profiles of MGMT in colorectal carcinoma surgical margins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical biochemistry

VOLUME: 41

Page Numbers: 19-25

Journal Abbreviation: Clin. Biochem.

ISSN: 0009-9120

DAY: 25

MONTH: 09

YEAR: 2007

Detailed methylation patterns and protein expression profiles of MGMT in colorectal carcinoma surgical margins. Information

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LANGUAGE: eng

NlmUniqueID: 133660

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Grant and Affiliation Information for Detailed methylation patterns and protein expression profiles of MGMT in colorectal carcinoma surgical margins.

AFFILIATION: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China; College of Animal Science and Technology, Jinling Institute of Technology, Nanjing 210038, China.

Country: United States

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MEDLINETA: Clin Biochem

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