Design and synthesis of a novel fluorescent reagent, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl) fluorescein, for carboxylic acids and its application in food samples using high-performance liquid chromatography.

Design and synthesis of a novel fluorescent reagent, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl) fluorescein, for carboxylic acids and its application in food samples using high-performance liquid chromatography. Research Abstract Details 

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Design and synthesis of a novel fluorescent reagent, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl) fluorescein, for carboxylic acids and its application in food samples using high-performance liquid chromatography. Abstract Text:

Xiao-Lan Du,Hua-Shan Zhang,Ying-Hua Deng,Hong Wang,

A novel derivatization reagent for carboxylic acids, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl)fluorescein (EPMF) has been well designed and synthesized. It was used to label 13 fatty acids, n-butyric acid (C4), n-valeric acid (C5), n-hexanoic acid (C6), n-heptanoic acid (C7), n-octanoic acid (C8), n-nonanoic acid (C9), n-decanoic acid (C10), lauric acid (C12), myristic acid (C14), palmitic acid (C16), stearic acid (C18), oleic acid (C18:1) and linoleic acid (C18:2), successfully. The derivatization reaction with fatty acids was completed at 50 degrees C, 50min. The derivatives of fatty acids were separated on a C18 reversed-phase column with gradient elution and fluorescence detection at lambda(ex)/lambda(em)=469/518nm. The detection limits obtained were 0.4-2nmolL(-1) (signal-to-noise ratio of 3). The proposed method has been applied to the quantification of fatty acids in edible oil with recoveries over 93%. It has been demonstrated that EPMF is a prominent derivatization reagent for fatty acids and is suitable for HPLC.

Design and synthesis of a novel fluorescent reagent, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl) fluorescein, for carboxylic acids and its application in food samples using high-performance liquid chromatography. Publishing Authors By Initials

XL Du,HS Zhang,YH Deng,H Wang,

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Design and synthesis of a novel fluorescent reagent, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl) fluorescein, for carboxylic acids and its application in food samples using high-performance liquid chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of chromatography. A

VOLUME: 1178

Page Numbers: 92-100

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ISSN: 0021-9673

DAY: 22

MONTH: 11

YEAR: 2007

Design and synthesis of a novel fluorescent reagent, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl) fluorescein, for carboxylic acids and its application in food samples using high-performance liquid chromatography. Information

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LANGUAGE: eng

NlmUniqueID: 9318488

Design and synthesis of a novel fluorescent reagent, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl) fluorescein, for carboxylic acids and its application in food samples using high-performance liquid chromatography. Keywords Mesh Terms:

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Grant and Affiliation Information for Design and synthesis of a novel fluorescent reagent, 6-oxy-(ethylpiperazine)-9-(2'-methoxycarbonyl) fluorescein, for carboxylic acids and its application in food samples using high-performance liquid chromatography.

AFFILIATION: Department of Chemistry, Wuhan University, Wuhan 430072, China.

Country: Netherlands

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MEDLINETA: J Chromatogr A

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