Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis.

Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis. Research Abstract Details 

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Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis. Abstract Text:

Cheng-Chung Chou,Konan Peck,Cheng-Chung Chou,Konan Peck,

DNA microarray technology has advanced rapidly since the first use of cDNA microarrays almost a decade ago. For gene expression studies on organisms, for which the genomes have been sequenced, cDNA microarrays are being gradually replaced by gene-specific oligonucleotide microarrays. Although, cDNA microarrays give higher signal intensity than oligonucleotide microarrays, they cannot be used for the measurement of gene-specific expression, whereas, oligonucleotide microarrays can. To obtain both a high signal intensity and specificity in gene expression measurements, gene-specific oligonucleotide probes as long as 150-mers, designed using sequence databases and algorithms to identify unique sequences of genes, are used as microarray probes. In order to achieve a high signal intensity, specificity, and accurate measurement of expression, in addition to the length and sequence of the probes, it is necessary to optimize other parameters such as the surface chemistry of the microarray slides, the addition of spacers and linkers to the probes, and the composition of the hybridization solution.

Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis. Publishing Authors By Initials

CC Chou,K Peck,CC Chou,K Peck,

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Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Methods in molecular biology (Clifton, N.J.)

VOLUME: 381

Page Numbers: 213-25

Journal Abbreviation: Methods Mol. Biol.

ISSN: 1064-3745

DAY: 6

MONTH: 11

YEAR: 2007

Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis. Information

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LANGUAGE: eng

NlmUniqueID: 9214969

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Grant and Affiliation Information for Design and fabrication of spotted long oligonucleotide microarrays for gene expression analysis.

AFFILIATION: Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi, Taiwan, Republic of China.

Country: United States

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MEDLINETA: Methods Mol Biol

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