Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring.

Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring. Research Abstract Details 

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Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring. Abstract Text:

Mehrdad Hajibabaei,Gregory A C Singer,Elizabeth L Clare,Paul D N Hebert,

BACKGROUND: The rapid and accurate identification of species is a critical component of large-scale biodiversity monitoring programs. DNA arrays (micro and macro) and DNA barcodes are two molecular approaches that have recently garnered much attention. Here, we compare these two platforms for identification of an important group, the mammals. RESULTS: Our analyses, based on the two commonly used mitochondrial genes cytochrome c oxidase I (the standard DNA barcode for animal species) and cytochrome b (a common species-level marker), suggest that both arrays and barcodes are capable of discriminating mammalian species with high accuracy. We used three different datasets of mammalian species, comprising different sampling strategies. For DNA arrays we designed three probes for each species to address intraspecific variation. As for DNA barcoding, our analyses show that both cytochrome c oxidase I and cytochrome b genes, and even smaller fragments of them (mini-barcodes) can successfully discriminate species in a wide variety of specimens. CONCLUSION: This study showed that DNA arrays and DNA barcodes are valuable molecular methods for biodiversity monitoring programs. Both approaches were capable of discriminating among mammalian species in our test assemblages. However, because designing DNA arrays require advance knowledge of target sequences, the use of this approach could be limited in large scale monitoring programs where unknown haplotypes might be encountered. DNA barcodes, by contrast, are sequencing-based and therefore could provide more flexibility in large-scale studies.

Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring. Publishing Authors By Initials

M Hajibabaei,GA Singer,EL Clare,PD Hebert,

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Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC biology

VOLUME: 5

Page Numbers: 24

Journal Abbreviation: BMC Biol.

ISSN: 1741-7007

DAY: 13

MONTH: 06

YEAR: 2007

Design and applicability of DNA arrays and DNA barcodes in biodiversity monitoring. Information

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LANGUAGE: eng

NlmUniqueID: 101190720

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AFFILIATION: Biodiversity Institute of Ontario, Department of Integrative Biology, University of Guelph, Guelph, Ontario, Canada. mhajibab@uoguelph.ca

Country: England

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MEDLINETA: BMC Biol

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