Depolarization-induced tyrosine phosphorylation in PC12h cells.

Depolarization-induced tyrosine phosphorylation in PC12h cells. Research Abstract Details 

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Depolarization-induced tyrosine phosphorylation in PC12h cells. Abstract Text:

N Okumura,M Okada,H Nakagawa,

Depolarization induced by KCl was found to induce tyrosine phosphorylation of cellular proteins in PC12h cells. By Western blotting with anti-phosphotyrosine antibody, we detected tyrosine phosphorylation of proteins with molecular weights of 120, 110, 105, 95, 75, 70, 66, 44, and 42 kDa in response to KCl. The immunoprecipitates from KCl-treated cells with the antibody contained large amounts of tyrosine-phosphorylated proteins and increased activity of tyrosine kinase. Incubation of the immunoprecipitates with [gamma-32P]ATP resulted in tyrosine phosphorylation of two proteins with the molecular weights of 120 and 140 kDa. These effects were completely abolished by the addition of EGTA before KCl treatment, suggesting that the depolarization-induced tyrosine phosphorylation may require calcium entry into the cells from the medium. Increased activity of tyrosine kinase phosphorylating the 120 and 140 kDa proteins was also recovered from cells stimulated with nerve growth factor, basic fibroblast growth factor, epidermal growth factor, and vasoactive intestinal peptide. Among them, depolarization by KCl elicited the strongest effect. These results indicate that a protein tyrosine kinase that phosphorylate the 120 and 140 kDa proteins is phosphorylated or activated in response to calcium ion, cAMP, and growth factors acting through tyrosine kinase receptors.

Depolarization-induced tyrosine phosphorylation in PC12h cells. Publishing Authors By Initials

N Okumura,M Okada,H Nakagawa,

For similar amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research abstracts see: amino acids, peptides, and proteins: amino acids: amino acids, cyclic: amino acids, aromatic: tyrosine research

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Depolarization-induced tyrosine phosphorylation in PC12h cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 116

Page Numbers: 346-50

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Aug

YEAR: 1994

Depolarization-induced tyrosine phosphorylation in PC12h cells. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Depolarization-induced tyrosine phosphorylation in PC12h cells. Keywords Mesh Terms:

KEYWORDS: Tyrosine

MESH TERMS: metabolism

Chemical & Substance for Abstract: Depolarization-induced tyrosine phosphorylation in PC12h cells. Information

Substance Name: Protein-Tyrosine Kinases

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Depolarization-induced tyrosine phosphorylation in PC12h cells.

AFFILIATION: Division of Metabolism, Osaka University.

Country: JAPAN

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MEDLINETA: J Biochem

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