Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters.

Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. Abstract Text:

Caryn Wadler,John E Cronan,

A new approach to determine in vivo pools of coenzyme A (CoA) and short chain acyl-CoA thioesters is reported. The metabolites released by extraction with trichloroacetic acid are recovered and quantitatively dephosphorylated by treatment with shrimp alkaline phosphatase. Following phosphatase removal, the dephosphorylated CoA metabolites are quantitatively rephosphorylated by treatment with [gamma-33P]ATP plus a dephospho-CoA kinase. The resulting radioactive CoA metabolites are then separated by reverse-phase high-performance liquid chromatography and quantitated by scintillation counting. Due to the enzymatic radiophosphorylation, the assay is specific for CoA and its short chain thioesters and is sensitive to sub-picomole levels of these compounds.

Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. Publishing Authors By Initials

C Wadler,JE Cronan,

For similar investigative techniques: radiometry: scintillation counting research abstracts see: investigative techniques: radiometry: scintillation counting research

PUBMED ID PMID:

MEDLINE DATE:

Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Analytical biochemistry

VOLUME: 368

Page Numbers: 17-23

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 7

MONTH: 06

YEAR: 2007

Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370535

Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. Keywords Mesh Terms:

KEYWORDS: Scintillation Counting

MESH TERMS: metabolism

Chemical & Substance for Abstract: Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters. Information

Substance Name: dephospho-CoA kinase

Registry Number: EC 2.7.1.24

Grant and Affiliation Information for Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters.

AFFILIATION: Department of Microbiology, University of Illinois, Urbana, IL 61801, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI15650

ACRONYM: AI

MEDLINETA: Anal Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters Related Publications

• Acyl carrier protein phosphodiesterase (AcpH) of Escherichia coli is a non-canonical member of the HD phosphatase/phosphodiesterase family.
• CT appearance of splenic injuries managed nonoperatively.
• Coordinate expression of the acetyl coenzyme A carboxylase genes, accB and accC, is necessary for normal regulation of biotin synthesis in Escherichia coli.
• Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters.
• Fourteen percent mortality rate in catheter-drainage series: setting the record straight.
• Genetic Interaction Between the Escherichia coli AcpT Phosphopantetheinyl Transferase and the YejM Inner Membrane Protein.
• Imaging-guided percutaneous radiofrequency ablation of solid renal masses: techniques and outcomes of 38 treatment sessions in 32 consecutive patients.
• In vivo functional analyses of the type II acyl carrier proteins of fatty acid biosynthesis.
• In vivo resolution of conflicting in vitro results: synthesis of biotin from dethiobiotin does not require pyridoxal phosphate.
• My first job: the transition from residency to employment-what the employer and employee should know.
• Radiology of male periurethral structures.
• Referrals to a dedicated cardiac MRI service: who sends what?
• The Lactococcus lactis FabF fatty acid synthetic enzyme can functionally replace both the FabB and FabF proteins of Escherichia coli and the FabH protein of Lactococcus lactis.
• Thyroid nodules: is it time to turn off the US machines?
• Understanding ethnic disparities in the use of total joint arthroplasty: Application of the health belief model.
• Vibrio cholerae FabV Defines a New Class of Enoyl-Acyl Carrier Protein Reductase.