Deoxyribozymes that synthesize branched and lariat RNA.

Deoxyribozymes that synthesize branched and lariat RNA. Research Abstract Details 

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Deoxyribozymes that synthesize branched and lariat RNA. Abstract Text:

Yangming Wang,Scott K Silverman,

Branched RNA molecules with a 2',5'-phosphodiester linkage are important biochemical intermediates. Lariat RNA is a particular type of branched RNA that is formed during intron splicing in vivo. Synthesis of branched and lariat RNA is challenging, and there are few general approaches that are applicable in vitro. Here we report the identification of divalent metal-dependent deoxyribozymes (DNA enzymes) that synthesize branched and lariat RNA. In vitro selection was used to obtain deoxyribozymes that selectively join an internal RNA 2'-hydroxyl with a 5'-terminal triphosphate in a convenient "binding arms" format. At least 85% yield of 2',5'-branched RNA is obtained at 37 degrees C and 20 mM Mn2+, pH 7.5 in

Deoxyribozymes that synthesize branched and lariat RNA. Publishing Authors By Initials

Y Wang,SK Silverman,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna research

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Deoxyribozymes that synthesize branched and lariat RNA. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 125

Page Numbers: 6880-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 11

MONTH: Jun

YEAR: 2003

Deoxyribozymes that synthesize branched and lariat RNA. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Deoxyribozymes that synthesize branched and lariat RNA. Keywords Mesh Terms:

KEYWORDS: RNA

MESH TERMS: chemical synthesis

Chemical & Substance for Abstract: Deoxyribozymes that synthesize branched and lariat RNA. Information

Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Deoxyribozymes that synthesize branched and lariat RNA.

AFFILIATION: Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.

Country: United States

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MEDLINETA: J Am Chem Soc

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