Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles.

Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles. Research Abstract Details 

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Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles. Abstract Text:

Harris Bernstein,Hana Holubec,Carol Bernstein,Natalia A Ignatenko,Eugene Gerner,Katerina Dvorak,David Besselsen,Karen Ann Blohm-Mangone,Jose Padilla-Torres,Barbora Dvorakova,Harinder Garewal,Claire M Payne,

Nos2 knockout mice were compared to wild-type mice for susceptibility to colitis in response to a diet supplemented with deoxycholate, a bile acid increased in the colon of individuals on a high-fat diet. Wild-type mice fed a fat-related diet, supplemented with 0.2% DOC, develop colonic inflammation associated with increases in nitrosative stress, proliferation, oxidative DNA/RNA damage, and angiogenesis, as well as altered expression of numerous genes. However, Nos2 knockout mice fed a diet supplemented with deoxycholate were resistant to these alterations. In particular, 35 genes were identified whose expression was significantly altered at the mRNA level in deoxycholate-fed Nos2(+/+) mice but not in deoxycholate-fed Nos2(-/-) mice. Some of these alterations in NOS2-dependent gene expression correspond to those reported in human inflammatory bowel disease. Overall, our results indicate that NOS2 expression is necessary for the development of deoxycholate-induced colitis in mice, a unique dietary-related model of colitis.

Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles. Publishing Authors By Initials

H Bernstein,H Holubec,C Bernstein,NA Ignatenko,E Gerner,K Dvorak,D Besselsen,KA Blohm-Mangone,J Padilla-Torres,B Dvorakova,H Garewal,CM Payne,

For similar cells: cellular structures: cell membrane: cell membrane structures: intercellular junctions: tight junctions research abstracts see: cells: cellular structures: cell membrane: cell membrane structures: intercellular junctions: tight junctions research

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Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Digestive diseases and sciences

VOLUME: 52

Page Numbers: 628-42

Journal Abbreviation:

ISSN: 0163-2116

DAY: 3

MONTH: Mar

YEAR: 2007

Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles. Information

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LANGUAGE: eng

NlmUniqueID: 7902782

Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles. Keywords Mesh Terms:

KEYWORDS: Tight Junctions

MESH TERMS: physiology

Chemical & Substance for Abstract: Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles. Information

Substance Name: Nitric Oxide Synthase

Registry Number: EC 1.14.13.39

Grant and Affiliation Information for Deoxycholate-induced colitis is markedly attenuated in Nos2 knockout mice in association with modulation of gene expression profiles.

AFFILIATION: Department of Cell Biology and Anatomy, College of Medicine, University of Arizona, Tucson, Arizona 85724-5044, USA. bernstein3@earthlink.net

Country: United States

AGENCY: United States NCI

GRANT: CA72008

ACRONYM: CA

MEDLINETA: Dig Dis Sci

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