Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells.

Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells. Research Abstract Details 

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Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells. Abstract Text:

Andrzej Myc, Majoros,Thommey P Thomas,James R Baker,

Our previous studies have demonstrated the applicability of poly(amidoamine) (PAMAM) dendrimers as a platform for the targeted delivery of chemotherapeutic drugs both in vitro and in vivo. To monitor the rate and extent of cell-killing caused by the delivered chemotherapeutic drug, we wished to analyze the degree of apoptosis in targeted cells on a real-time basis. As the apoptosis-regulating caspases are activated during the apoptotic process, several caspase-hydrolyzable, fluorescence resonance energy transfer (FRET)-based substrates have been marketed for the detection of apoptosis. However, the applicability of these agents is limited because of their nonspecificity and the consequent high background fluorescence in tissues. Here we show the synthesis, characterization, and in vitro targeting of an engineered PAMAM nanodevice in which folic acid (FA) is conjugated as the targeting molecule and a caspase-specific FRET-based agent (PhiPhiLux G1D2) is conjugated as the apoptosis-detecting agent. This conjugate specifically targets FA-receptor-positive, KB cells. In these cells, the apoptosis-inducing agent staurosporine caused a 5-fold increase in the cellular fluorescence. These results show, for the first time, the potential applicability of a targeted apoptosis-measuring nanodevice, which could be used for simultaneously monitoring the apoptotic potential of a delivered drug.

Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells. Publishing Authors By Initials

A Myc,IJ Majoros,TP Thomas,JR Baker,

For similar neoplasms research abstracts see: neoplasms research

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Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Biomacromolecules

VOLUME: 8

Page Numbers: 13-8

Journal Abbreviation: Biomacromolecules

ISSN: 1525-7797

DAY: 3

MONTH: Jan

YEAR: 2007

Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100892849

Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells. Keywords Mesh Terms:

KEYWORDS: Neoplasms

MESH TERMS: pathology

Chemical & Substance for Abstract: Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells. Information

Substance Name: Dendrimers

Registry Number: 0

Grant and Affiliation Information for Dendrimer-based targeted delivery of an apoptotic sensor in cancer cells.

AFFILIATION: Michigan Nanotechnology Institute for Medicine and Biological Sciences, University of Michigan, Ann Arbor, Michigan 48109, USA.

Country: United States

AGENCY: United States NCI

GRANT: N01-CM-97065-32

ACRONYM: CM

MEDLINETA: Biomacromolecules

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