Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis.

Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis. Research Abstract Details 

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Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis. Abstract Text:

Kandice R Johnson,Jennifer L Leight,Valerie M Weaver,

Tissue morphogenesis and homeostasis are dependent on a complex dialogue between multiple cell types and chemical and physical cues in the surrounding microenvironment. The emergence of engineered three-dimensional (3D) tissue constructs and the development of tractable methods to recapitulate the native tissue microenvironment ex vivo has led to a deeper understanding of tissue-specific behavior. However, much remains unclear about how the microenvironment and aberrations therein directly affect tissue morphogenesis and behavior. Elucidating the role of the microenvironment in directing tissue-specific behavior will aid in the development of surrogate tissues and tractable approaches to diagnose and treat chronic-debilitating diseases such as cancer and atherosclerosis. Toward this goal, 3D organotypic models have been developed to clarify the mechanisms of epithelial morphogenesis and the subsequent maintenance of tissue homeostasis. Here we describe the application of these 3D culture models to illustrate how the microenvironment plays a critical role in regulating mammary tissue function and signaling, and discuss the rationale for applying precisely defined organotypic culture assays to study epithelial cell behavior. Experimental methods are provided to generate and manipulate 3D organotypic cultures to study the effect of matrix stiffness and matrix dimensionality on epithelial tissue morphology and signaling. We end by discussing technical limitations of currently available systems and by presenting opportunities for improvement.

Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis. Publishing Authors By Initials

KR Johnson,JL Leight,VM Weaver,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Methods in cell biology

VOLUME: 83

Page Numbers: 547-83

Journal Abbreviation: Methods Cell Biol.

ISSN: 0091-679X

DAY: 3

MONTH: 12

YEAR: 2007

Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis. Information

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LANGUAGE: eng

NlmUniqueID: 373334

Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: isolation & purification

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Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for Demystifying the effects of a three-dimensional microenvironment in tissue morphogenesis.

AFFILIATION: Institute for Medicine and Engineering, Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: T32HL00795404

ACRONYM: HL

MEDLINETA: Methods Cell Biol

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