Demonstration of type II hemidesmosomes in a mammary gland epithelial cell line, BMGE-H.

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Demonstration of type II hemidesmosomes in a mammary gland epithelial cell line, BMGE-H. Abstract Text:

J Uematsu,Y Nishizawa,A Sonnenberg,K Owaribe,

Hemidesmosomes (HDs) are specialized cell-substrate junctions with distinct cytoplasmic plaques where intermediate filaments (IFs) are anchored. In our previous work, we described two types of HDs in terms of their molecular constituents, i.e. type I HD and type II HD [Hieda, Y., Nishizawa, Y., Uematsu, J., & Owaribe, K. (1992) J. Cell Biol. 116, 1497-1506]. In the present study we further characterized type II HDs in cultured cells, comparing their composition and function with those of conventional type I HDs. Although the two bovine mammary gland epithelial cell lines, BMGE+H and BMGE-H, were derived from the same tissue, their cell-substrate adhesion properties are markedly different. Immunological examination showed that BMGE-H cells express HD1 and the integrin alpha 6 beta 4 complex but not bullous pemphigoid antigens, while BMGE+H cells express all these components, i.e. the former have type II HDs and the latter have type I HDs. GoH3, a monoclonal antibody to the integrin alpha 6 subunit, inhibited BMGE-H cell adhesion to laminin as a substrate, as also observed for BMGE+H cells. These and electron microscopic results indicate that BMGE-H cells form type II HD-like structures containing HD1 and alpha 6 beta 4 which are associated with IFs. This structure mediates adhesion of the cell to laminin. This is the first demonstration of an adhesion function for type II HDs in cultured cells.

Demonstration of type II hemidesmosomes in a mammary gland epithelial cell line, BMGE-H. Publishing Authors By Initials

J Uematsu,Y Nishizawa,A Sonnenberg,K Owaribe,

For similar diagnosis: laboratory techniques and procedures: immunologic tests: serologic tests: precipitin tests research abstracts see: diagnosis: laboratory techniques and procedures: immunologic tests: serologic tests: precipitin tests research

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Demonstration of type II hemidesmosomes in a mammary gland epithelial cell line, BMGE-H. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 115

Page Numbers: 469-76

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1994

Demonstration of type II hemidesmosomes in a mammary gland epithelial cell line, BMGE-H. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Demonstration of type II hemidesmosomes in a mammary gland epithelial cell line, BMGE-H. Keywords Mesh Terms:

KEYWORDS: Precipitin Tests

MESH TERMS: immunology

Chemical & Substance for Abstract: Demonstration of type II hemidesmosomes in a mammary gland epithelial cell line, BMGE-H. Information

Substance Name: Collagen

Registry Number: 9007-34-5

Grant and Affiliation Information for Demonstration of type II hemidesmosomes in a mammary gland epithelial cell line, BMGE-H.

AFFILIATION: Department of Molecular Biology, School of Science, Nagoya University.

Country: JAPAN

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MEDLINETA: J Biochem

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