Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects.

Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Research Abstract Details 

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Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Abstract Text:

O B Betz,V M Betz,A Nazarian,M Egermann,L C Gerstenfeld,T A Einhorn,M S Vrahas,M L Bouxsein,C H Evans,

The direct, local, administration of adenovirus carrying human BMP-2 cDNA (Ad.BMP-2) heals critical-sized femoral bone defects in rabbit and rat models. However, the outcome is suboptimal and the technology needs to provide a more reliable and uniform outcome. To this end, we investigated whether the timing of Ad.BMP-2 administration influenced the formation of mineralized tissue within the defect. Critical-sized defects were created in the femora of 28 Sprague-Dawley rats. Animals were injected intralesionally with a single, percutaneous injection of Ad.BMP-2 (4 x 10(8) plaque-forming units) either intraoperatively (day 0) or 24 h (day 1), 5 days or 10 days after surgery. The femora were evaluated 8 weeks after surgery by X-ray, microcomputed tomography, dual-energy X-ray absorptiometry and biomechanical testing. The incidence of radiological union was markedly increased when administration of Ad.BMP-2 was delayed until days 5 and 10, at which point 86% of the defects healed. These time points also provided greater bone mineral content within the defect site and improved the average mechanical strength of the healed bone. Thus, delaying the injection of Ad.BMP-2 until 5 or 10 days after surgery enables a greater percentage of critical-sized, segmental defects to achieve radiological union, producing a repair tissue with enhanced mineralization and greater mechanical strength.

Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Publishing Authors By Initials

OB Betz,VM Betz,A Nazarian,M Egermann,LC Gerstenfeld,TA Einhorn,MS Vrahas,ML Bouxsein,CH Evans,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gene therapy

VOLUME: 14

Page Numbers: 1039-44

Journal Abbreviation: Gene Ther.

ISSN: 0969-7128

DAY: 26

MONTH: 04

YEAR: 2007

Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9421525

Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Information

Substance Name: bone morphogenetic protein 2

Registry Number: 0

Grant and Affiliation Information for Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects.

AFFILIATION: Center for Molecular Orthopaedics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Country: England

AGENCY: United States NIAMS

GRANT: AR 050243-02

ACRONYM: AR

MEDLINETA: Gene Ther

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